LOCUS       sequence-84942-e        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 399, Emission: 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: mT-Sapphire-C1
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..107
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     58..78
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      146..155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(152..154,155..157,158..868)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(197..218)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(458..477)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     805..826
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    869..934
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
       61 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      121 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
      181 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      241 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      301 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttctcct acggcgtgat
      361 ggtgttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      421 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      481 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      541 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
      601 cgtctatatc atggccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      661 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      721 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc atccagtcca agctgagcaa
      781 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      841 cactctgggc atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgaa
      901 ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
      961 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     1021 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     1081 tggttacaa
//
