LOCUS       sequence-84960-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mCherry2-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..67
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     18..38
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..819
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(257..275)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    820..885
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
       61 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagc
      121 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      181 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      241 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
      301 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      361 atccccgact acttgaagct gtccttcccc gagggcttca attgggagcg cgtgatgaac
      421 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
      481 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagtgt
      541 cgtaccatgg gctgggaggc ctccactgag cggatgtacc ccgaggacgg cgccctgaag
      601 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
      661 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt cgacatcaag
      721 ttggacatcc tttcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
      781 ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggactcag atctcgagct
      841 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa
      901 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
      961 cacctccccc tgaacctgaa acataaaatg aatg
//
