LOCUS       sequence-84999-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..21
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        6..50
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..135
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             157..867
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
       61 accgtcagat ccgctagcgc caccatgggc gtggccgact tgatcaagaa gttcgagtcc
      121 atctccaagg aggaggggga tccaccggtc gccaccatgg tgtctaaggg cgaagagctg
      181 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
      241 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
      301 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
      361 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
      421 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
      481 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
      541 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
      601 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
      661 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
      721 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
      781 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
      841 cctagcaaac tggggcacaa gcttaattaa gcggccgcga ctctagatca taatcagcca
      901 taccacatt
//
