LOCUS       sequence-85013-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 453, Emission: 486.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AmCyan1-N1
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..73
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     24..44
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    96..176
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      178..187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             184..873
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKKTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHVVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
ORIGIN
        1 cgtaacaact ccgcccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
       61 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc
      121 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
      181 accatggccc tgtccaacaa gttcatcggc gacgacatga agatgaccta ccacatggac
      241 ggctgcgtga acggccacta cttcaccgtg aagggcgagg gcagcggcaa gccctacgag
      301 ggcacccaga cctccacctt caaggtgacc atggccaacg gcggccccct ggccttctcc
      361 ttcgacatcc tgtccaccgt gttcatgtac ggcaaccgct gcttcaccgc ctaccccacc
      421 agcatgcccg actacttcaa gcaggccttc cccgacggca tgtcctacga gagaaccttc
      481 acctacgagg acggcggcgt ggccaccgcc agctgggaga tcagcctgaa gggcaactgc
      541 ttcgagcaca agtccacctt ccacggcgtg aacttccccg ccgacggccc cgtgatggcc
      601 aagaagacca ccggctggga cccctccttc gagaagatga ccgtgtgcga cggcatcttg
      661 aagggcgacg tgaccgcctt cctgatgctg cagggcggcg gcaactacag atgccagttc
      721 cacacctcct acaagaccaa gaagcccgtg accatgcccc ccaaccacgt ggtggagcac
      781 cgcatcgcca gaaccgacct ggacaagggc ggcaacagcg tgcagctgac cgagcacgcc
      841 gtggcccaca tcacctccgt ggtgcccttc taagcggccg cgactctaga tcataatcag
      901 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctccacacct ccccctgaac
      961 ctgaa
//
