LOCUS       sequence-85045-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: YPet-C1
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    752..817
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaaaggcg aagagctgtt
       61 caccggcgtg gtgcccatcc tggtggagct ggacggcgac gtgaacggcc acaagttcag
      121 cgtgagcggc gagggcgagg gcgacgccac ctacggcaag ctgaccctga agctgctgtg
      181 caccaccggc aagctgcccg tgccctggcc caccctggtg accaccctgg gctacggcgt
      241 gcagtgcttc gcccggtacc ccgaccacat gaagcagcac gacttcttca agagcgccat
      301 gcccgagggc tacgtgcagg agcggaccat cttcttcaag gacgacggca actacaagac
      361 ccgggccgag gtgaagttcg agggcgacac cctggtgaac cggatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg ccacaagctg gagtacaact acaacagcca
      481 caacgtgtac atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      541 gcacaacatc gaggacggcg gcgtgcagct ggccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccaga gcgccctgtt
      661 caaggacccc aacgagaagc gggaccacat ggtgctgctg gagttcctga ccgccgccgg
      721 catcaccgag ggcatgaacg agctctataa gtccggactc agatctcgag ctcaagcttc
      781 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
      841 aatcagccat accacatttg tagaggtttt acttgcttta aaaacctccc acacctcccc
      901 ctgaacctga aacataaatg aatgcaattg tgttgttaac ttgtttattg cagcttatat
      961 gatacaataa gcatagcatc acaaattt
//
