LOCUS       sequence-8519-ex       12546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   McKenzie GJ, Craig NL
  TITLE     Fast, easy and efficient: site-specific insertion of transgenes into
            enterobacterial chromosomes using Tn7 without need for selection of 
            the insertion event.
  JOURNAL   BMC Microbiol. 2006 Apr 28;6:39. doi: 10.1186/1471-2180-6-39.
  PUBMED    16646962
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2006-04-28"; volume: "6:39. doi"; pages: " 
            10.1186/1471-2180-6-39"
COMMENT     Sequence Label: pGRG25
FEATURES             Location/Qualifiers
     source          1..12546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(146..1024)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1051..1335
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1258..1277
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(8026..8043)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8196..8282
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8374..8401
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        8420..8511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8512..8580,8581..9372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8730..8749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(9501..9520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            9543..9652
                     /note="incP origin of transfer"
     primer_bind     complement(9690..9709)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      10392..10614
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             10662..11612
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  12045..12210
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatctg ctcatgtttg acagcttatc atcgatgcat
       61 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc
      121 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt
      181 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      241 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      301 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      361 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      421 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      481 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      541 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      601 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      661 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      721 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      781 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      841 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      901 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      961 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1021 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1081 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1141 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1201 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1261 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     1321 ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt acccaagtct
     1381 taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac
     1441 tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt
     1501 attaaagagg ggcgtggcca agggcatggt aaagactata ttccatggct aacagtacaa
     1561 gaagttcctt cttcaggtcg ttcccaccgt atttattctc ataagacggg acgagtccat
     1621 catttgctat ctgacttaga gcttgctgtt tttctcagtc ttgagtggga gagcagcgtg
     1681 ctagatatac gcgagcagtt ccccttatta cctagtgata ccaggcagat tgcaatagat
     1741 agtggtatta agcatcctgt tattcgtggt gtagatcagg ttatgtctac tgatttttta
     1801 gtggactgca aagatggtcc ttttgagcag tttgctattc aagtcaaacc tgcagcagcc
     1861 ttacaagacg agcgtacctt agaaaaacta gaactagagc gtcgctattg gcagcaaaag
     1921 caaattcctt ggttcatttt tactgataaa gaaataaatc ccgtagtaaa agaaaatatt
     1981 gaatggcttt attcagtgaa aacagaagaa gtttctgcgg agcttttagc acaactatcc
     2041 ccattggccc atatcctgca agaaaaagga gatgaaaaca ttatcaatgt ctgtaagcag
     2101 gttgatattg cttatgattt ggagttaggc aaaacattga gtgagatacg agccttaacc
     2161 gcaaatggtt ttattaagtt caatatttat aagtctttca gggcaaataa gtgtgcagat
     2221 ctctgtatta gccaagtagt gaatatggag gagttgcgct atgtggcaaa ttaatgaggt
     2281 tgtgctattt gataatgatc cgtatcgcat tttggctata gaggatggcc aagttgtctg
     2341 gatgcaaata agcgctgata aaggagttcc acaagctagg gctgagttgt tgctaatgca
     2401 gtatttagat gaaggccgct tagttagaac tgatgaccct tatgtacatc ttgatttaga
     2461 agagccgtct gtagattctg tcagcttcca gaagcgcgag gaggattatc gaaaaattct
     2521 tcctattatt aatagtaagg atcgtttcga ccctaaagtc agaagcgaac tcgttgagca
     2581 tgtggtccaa gaacataagg ttactaaggc tacagtttat aagttgttac gccgttactg
     2641 gcagcgtggt caaacgccta atgcattaat tcctgactac aaaaacagcg gtgcaccagg
     2701 ggaaagacgt tcagcgacag gaacagcaaa gattggccga gccagagaat atggtaaggg
     2761 tgaaggaacc aaggtaacgc ccgagattga acgccttttt aggttgacca tagaaaagca
     2821 cctgttaaat caaaaaggta caaagaccac cgttgcctat agacgatttg tggacttgtt
     2881 tgctcagtat tttcctcgca ttccccaaga ggattaccca acactacgtc agtttcgtta
     2941 tttttatgat cgagaatacc ctaaagctca gcgcttaaag tctagagtta aagcaggggt
     3001 atataaaaaa gacgtacgac ccttaagtag tacagccact tctcaggcgt taggccctgg
     3061 gagtcgttat gagattgatg ccacgattgc tgatatttat ttagtggatc atcatgatcg
     3121 ccaaaaaatc ataggaagac caacgcttta cattgtgatt gatgtgttta gtcggatgat
     3181 cacgggcttt tatatcggct ttgaaaatcc gtcttatgtg gtggcgatgc aggcttttgt
     3241 aaatgcttgc tctgacaaaa cggccatttg tgcccagcat gatattgaga ttagtagctc
     3301 agactggccg tgtgtaggtt tgccagatgt gttgctagcg gaccgtggcg aattaatgag
     3361 tcatcaggtc gaagccttag tttctagttt taatgtgcga gtggaaagtg ctccacctag
     3421 acgtggcgat gctaaaggca tagtggaaag cacttttaga acactacaag ccgagtttaa
     3481 gtcctttgca cctggcattg tagagggcag tcggatcaaa agccatggtg aaacagacta
     3541 taggttagat gcatctctgt cggtatttga gttcacacaa attattttgc gtacgatctt
     3601 attcagaaat aaccatctgg tgatggataa atacgatcga gatgctgatt ttcctacaga
     3661 tttaccgtct attcctgtcc agctatggca atggggtatg cagcatcgta caggtagttt
     3721 aagggctgtg gagcaagagc agttgcgagt agcgttactg cctcgccgaa aggtctctat
     3781 ttcttcattt ggcgttaatt tgtggggttt gtattactcg gggtcagaga ttctgcgtga
     3841 gggttggttg cagcggagca ctgatatagc tagacctcaa catttagaag cggcttatga
     3901 cccagtgctg gttgatacga tttatttgtt tccgcaagtt ggcagccgtg tattttggcg
     3961 ctgtaatctg acggaacgta gtcggcagtt taaaggtctc tcattttggg aggtttggga
     4021 tatacaagca caagaaaaac acaataaagc caatgcgaag caggatgagt taactaaacg
     4081 cagggagctt gaggcgttta ttcagcaaac cattcagaaa gcgaataagt taacgcccag
     4141 tactactgag cccaaatcaa cacgcattaa gcagattaaa actaataaaa aagaagccgt
     4201 gacctcggag cgtaaaaaac gtgcggagca tttgaagcca agctcttcag gtgatgaggc
     4261 taaagttatt cctttcaacg cagtggaagc ggatgatcaa gaagattaca gcctacccac
     4321 atacgtgcct gaattatttc aggatccacc agaaaaggat gagtcatgag tgctacccgg
     4381 attcaagcag tttatcgtga tacgggggta gaggcttatc gtgataatcc ttttatcgag
     4441 gccttaccac cattacaaga gtcagtgaat agtgctgcat cactgaaatc ctctttacag
     4501 cttacttcct ctgacttgca aaagtcccgt gttatcagag ctcataccat ttgtcgtatt
     4561 ccagatgact attttcagcc attaggtacg catttgctac taagtgagcg tatttcggtc
     4621 atgattcgag gtggctacgt aggcagaaat cctaaaacag gagatttaca aaagcattta
     4681 caaaatggtt atgagcgtgt tcaaacggga gagttggaga catttcgctt tgaggaggca
     4741 cgatctacgg cacaaagctt attgttaatt ggttgttctg gtagtgggaa gacgacctct
     4801 cttcatcgta ttctagccac gtatcctcag gtgatttacc atcgtgaact caatgtagag
     4861 caggtggtgt atttgaaaat agactgctcg cataatggtt cgctaaaaga aatctgcttg
     4921 aattttttca gagcgttgga tcgagccttg ggctcgaact atgagcgtcg ttatggctta
     4981 aaacgtcatg gtatagaaac catgttggct ttgatgtcgc aaatagccaa tgcacatgct
     5041 ttagggttgt tggttattga tgaaattcag catttaagcc gctctcgttc gggtggatct
     5101 caagagatgc tgaacttttt tgtgacgatg gtgaatatta ttggcgtacc agtgatgttg
     5161 attggtaccc ctaaagcacg agagattttt gaggctgatt tgcggtctgc acgtagaggg
     5221 gcagggtttg gagctatatt ctgggatcct atacaacaaa cgcaacgtgg aaagcccaat
     5281 caagagtgga tcgcttttac ggataatctt tggcaattac agcttttaca acgcaaagat
     5341 gcgctgttat cggatgaggt ccgtgatgtg tggtatgagc taagccaagg agtgatggac
     5401 attgtagtaa aactttttgt actcgctcag ctccgtgcgc tagctttagg caatgagcgt
     5461 attaccgctg gtttattgcg gcaagtgtat caagatgagt taaagcctgt gcaccccatg
     5521 ctagaggcat tacgctcggg tatcccagaa cgcattgctc gttattctga tctagtcgtt
     5581 cccgagattg ataaacggtt aatccaactt cagctagata tcgcagcgat acaagaacaa
     5641 acaccagaag aaaaagccct tcaagagtta gataccgaag atcagcgtca tttatatctg
     5701 atgctgaaag aggattacga ttcaagcctg ttaattccca ctattaaaaa agcgtttagc
     5761 cagaatccaa cgatgacaag acaaaagtta ctgcctcttg ttttgcagtg gttgatggaa
     5821 ggcgaaacgg tagtgtcaga actagaaaag ccctccaaga gtaaaaaggt ttcggctata
     5881 aaggtagtca agcccagcga ctgggatagc ttgcctgata cggatttacg ttatatctat
     5941 tcacaacgcc aacctgaaaa aaccatgcat gaacggttaa aagggaaagg ggtaatagtg
     6001 gatatggcga gcttatttaa acaagcaggt tagccatgag aaactttcct gttccgtact
     6061 cgaatgagct gatttatagc actattgcac gggcaggcgt ttatcaaggg attgttagtc
     6121 ctaagcagct gttggatgag gtgtatggca accgcaaggt ggtcgctacc ttaggtctgc
     6181 cctcgcattt aggtgtgata gcaagacatc tacatcaaac aggacgttac gctgttcagc
     6241 agcttattta tgagcatacc ttattccctt tatatgctcc gtttgtaggc aaggagcgcc
     6301 gagacgaagc tattcggtta atggagtacc aagcgcaagg tgcggtgcat ttaatgctag
     6361 gagtcgctgc ttctagagtt aagagcgata accgctttag atactgccct gattgcgttg
     6421 ctcttcagct aaataggtat ggggaagcct tttggcaacg agattggtat ttgcccgctt
     6481 tgccatattg tccaaaacac ggtgctttag tcttctttga tagagctgta gatgatcacc
     6541 gacatcaatt ttgggctttg ggtcatactg agctgctttc agactacccc aaagactccc
     6601 tatctcaatt aacagcacta gctgcttata tagcccctct gttagatgct ccacgagcgc
     6661 aagagctttc cccaagcctt gagcagtgga cgctgtttta tcagcgctta gcgcaggatc
     6721 tagggctaac caaaagcaag cacattcgtc atgacttggt ggcggagaga gtgaggcaga
     6781 cttttagtga tgaggcacta gagaaactgg atttaaagtt ggcagagaac aaggacacgt
     6841 gttggctgaa aagtatattc cgtaagcata gaaaagcctt tagttattta cagcatagta
     6901 ttgtgtggca agccttattg ccaaaactaa cggttataga agcgctacag caggcaagtg
     6961 ctcttactga gcactctata acgacaagac ctgttagcca gtctgtgcaa cctaactctg
     7021 aagatttatc tgttaagcat aaagactggc agcaactagt gcataaatac caaggaatta
     7081 aggcggcaag acagtcttta gagggtgggg tgctatacgc ttggctttac cgacatgaca
     7141 gggattggct agttcactgg aatcaacagc atcaacaaga gcgtctggca cccgccccta
     7201 gagttgattg gaaccaaaga gatcgaattg ctgtacgaca actattaaga atcataaagc
     7261 gtctagatag tagccttgat cacccaagag cgacatcgag ctggctgtta aagcaaactc
     7321 ctaacggaac ctctcttgca aaaaatctac agaaactgcc tttggtagcg ctttgcttaa
     7381 agcgttactc agagagtgtg gaagattatc aaattagacg gattagccaa gcttttatta
     7441 agcttaaaca ggaagatgtt gagcttaggc gctggcgatt attaagaagt gcaacgttat
     7501 ctaaagagcg gataactgag gaagcacaaa gattcttgga aatggtttat ggggaagagt
     7561 gagtggttag gctagctaca tttaatgaca atgtgcaggt tgtacatatt ggtcatttat
     7621 tccgtaactc gggtcataag gagtggcgta tttttgtttg gtttaatcca atgcaagaac
     7681 ggaaatggac tcgatttact catttgcctt tattaagtcg agctaaggtg gttaacagta
     7741 caacaaagca aataaataag gcggatcgtg tgattgagtt tgaagcatcg gatcttcaac
     7801 gagccaaaat aatcgatttt cctaatctct cgtcctttgc ttccgtacgc aacaaggatg
     7861 gagcgcagag ttcatttatt tacgaagctg aaacaccata tagcaagact cgttatcaca
     7921 tcccacagtt agagctagct cggtcattat ttttagggga tcctctagag tcgacctgca
     7981 ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa
     8041 atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt
     8101 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
     8161 gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     8221 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     8281 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     8341 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     8401 ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8461 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8521 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8581 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8641 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8701 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     8761 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8821 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8881 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8941 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9001 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     9061 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9121 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9181 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9241 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9301 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9361 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc
     9421 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     9481 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     9541 cgccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
     9601 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggcggcat
     9661 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     9721 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     9781 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     9841 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     9901 gcgtcgatcc ccgacagtaa gacgggtaag cctgttgatg ataccgctgc cttactgggt
     9961 gcattagcca gtctgaatga cctgtcacgg gataatccga agtggtcaga ctggaaaatc
    10021 agagggcagg aactgctgaa cagcaaaaag tcagatagca ccacatagca gacccgccat
    10081 aaaacgccct gagaagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg
    10141 aatccataaa aggcgcctgt agtgccattt acccccattc actgccagag ccgtgagcgc
    10201 agcgaactga atgtcacgaa aaagacagcg actcaggtgc ctgatggtcg gagacaaaag
    10261 gaatattcag cgatttgccc gagcttgcga gggtgctact taagccttta gggttttaag
    10321 gtctgttttg tagaggagca aacagcgttt gcgacatcct tttgtaatac tgcggaactg
    10381 actaaagtag tgagttatac acagggctgg gatctattct ttttatcttt ttttattctt
    10441 tctttattct ataaattata accacttgaa tataaacaaa aaaaacacac aaaggtctag
    10501 cggaatttac agagggtcta gcagaattta caagttttcc agcaaaggtc tagcagaatt
    10561 tacagatacc cacaactcaa aggaaaagga ctagtaatta tcattgacta gcccatctca
    10621 attggtatag tgattaaaat cacctagacc aattgagatg tatgtctgaa ttagttgttt
    10681 tcaaagcaaa tgaactagcg attagtcgct atgacttaac ggagcatgaa accaagctaa
    10741 ttttatgctg tgtggcacta ctcaacccca cgattgaaaa ccctacaagg aaagaacgga
    10801 cggtatcgtt cacttataac caatacgttc agatgatgaa catcagtagg gaaaatgctt
    10861 atggtgtatt agctaaagca accagagagc tgatgacgag aactgtggaa atcaggaatc
    10921 ctttggttaa aggctttgag attttccagt ggacaaacta tgccaagttc tcaagcgaaa
    10981 aattagaatt agtttttagt gaagagatat tgccttatct tttccagtta aaaaaattca
    11041 taaaatataa tctggaacat gttaagtctt ttgaaaacaa atactctatg aggatttatg
    11101 agtggttatt aaaagaacta acacaaaaga aaactcacaa ggcaaatata gagattagcc
    11161 ttgatgaatt taagttcatg ttaatgcttg aaaataacta ccatgagttt aaaaggctta
    11221 accaatgggt tttgaaacca ataagtaaag atttaaacac ttacagcaat atgaaattgg
    11281 tggttgataa gcgaggccgc ccgactgata cgttgatttt ccaagttgaa ctagatagac
    11341 aaatggatct cgtaaccgaa cttgagaaca accagataaa aatgaatggt gacaaaatac
    11401 caacaaccat tacatcagat tcctacctac ataacggact aagaaaaaca ctacacgatg
    11461 ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa atttttgagt gacatgcaaa
    11521 gtaagtatga tctcaatggt tcgttctcat ggctcacgca aaaacaacga accacactag
    11581 agaacatact ggctaaatac ggaaggatct gaggttctta tggctcttgt atctatcagt
    11641 gaagcatcaa gactaacaaa caaaagtaga acaactgttc accgttacat atcaaaggga
    11701 aaactgtcca tatgcacaga tgaaaacggt gtaaaaaaga tagatacatc agagctttta
    11761 cgagtttttg gtgcatttaa agctgttcac catgaacaga tcgacaatgt aacagatgaa
    11821 cagcatgtaa cacctaatag aacaggtgaa accagtaaaa caaagcaact agaacatgaa
    11881 attgaacacc tgagacaact tgttacagct caacagtcac acatagacag cctgaaacag
    11941 gcgatgctgc ttatcgaatc aaagctgccg acaacacggg agccagtgac gcctcccgtg
    12001 gggaaaaaat catggcaatt ctggaagaaa tagcgctttc agcctgtggg cggacaaaat
    12061 agttgggaac tgggaggggt ggaaatggag tttttaagga ttatttaggg aagagtgaca
    12121 aaatagatgg gaactgggtg tagcgtcgta agctaatacg aaaattaaaa atgacaaaat
    12181 agtttggaac tagatttcac ttatctggtt ggtcgacact agtattaccc tgttatccct
    12241 agatttaaat gatatcggat cctagtaagc cacgttttaa ttaatcagat ccctcaatag
    12301 cggccgccac tcgagcacct aggaggcgcg ccacggccgc gtcgacccca cgcccctctt
    12361 taatacgacg ggcaatttgc acttcagaaa atgaagagtt tgctttagcc ataacaaaag
    12421 tccagtatgc tttttcacag cataactgga ctgatttcag tttacaacta ttctgtctag
    12481 tttaagactt tattgtcata gtttagatct attttgttca gtttaagact ttattgtccg
    12541 cccaca
//
