LOCUS       sequence-85200-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 
            500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: mCherry-miniSOG-C1
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..349
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(501..519)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     769..788
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggagaag agcttcgtga tcaccgaccc
       61 ccgcctgccc gacaacccca tcatcttcgc cagcgacggc ttcctggagc tgaccgagta
      121 cagcagagag gagatcctgg gccgcaacgg ccgcttcctg cagggccccg agaccgacca
      181 ggccaccgtg cagaagatcc gcgacgccat ccgcgaccag cgcgagatca ccgtgcagct
      241 gatcaactac accaagagcg gcaagaagtt ctggaacctg ctgcacctgc agcccatgcg
      301 cgaccagaag ggcgagctgc agtacttcat cggcgtgcag ctggacggcg gcggcatggt
      361 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      421 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      481 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
      541 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
      601 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      661 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
      721 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
      781 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
      841 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
      901 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
      961 caagttggac atcacctccc acaacg
//
