LOCUS       sequence-85212-e        1255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos-M-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..1255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..89
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             111..791
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    913..994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(950..969)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1004..1023
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accaatggct tagtgacgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca
       61 agaagttcga gtccatctcc aaggaggagg gggatccacc ggtcgccacc atgagtgcga
      121 ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg caccactttg
      181 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
      241 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttcatgtacg
      301 gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag cagtcgtttc
      361 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcattgcca
      421 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt tatggtacca
      481 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
      541 agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat tcatatggct ttgttgcttg
      601 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
      661 tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc catgacaaag
      721 attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg
      781 ccagacgata agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
      841 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      901 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      961 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1021 atcaatgtat tcttaaggcg taaattgtaa gcgttaatat ttgttaaaat tcgcgttaaa
     1081 ttttgtaaat cagctcattt ttacatagcg aatcgcaatc ctataaatca aagaatagac
     1141 gagataggtg agtgtgtcag tttggaacaa gagtcactat tagacggtga tccagctaag
     1201 gctaaatcgt ctattcaggg cgagtgctac tacgtgtaac catctacacc ctatg
//
