LOCUS       sequence-85213-e        1272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos-M-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..1272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        230..433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     384..404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      462..471
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             468..518
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             540..1220
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ccgcgcccaa ttgaagcgct caatataagg cgtatgttcc atagtacgcc aatagactcc
       61 atgagtcatg gtggagtatt taacgtactg cccactgcag tacatcagtg ttatcattgc
      121 agtacgcccc tattgacgtc atgacgtaaa tgaccgctgc attatgccca gtacatgact
      181 atggactttc tactggcagt acatctacgt atagtcatcg ctattacatg tgatgcggtt
      241 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      301 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      361 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      421 tataagcaga gctggtttag tgaaccgtca gatccgctag cgccaccatg ggcgtggccg
      481 acttgatcaa gaagttcgag tccatctcca aggaggaggg ggatccaccg gtcgccacca
      541 tgagtgcgat taagccagac atgaagatca aactccgtat ggaaggcaac gtaaacgggc
      601 accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa cagagtatgg
      661 atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg accactgcat
      721 tcatgtacgg caacagggta ttcgccaaat atccagacaa catacaagac tattttaagc
      781 agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac gggggcattt
      841 gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa gttcgatttt
      901 atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg aaatgggagc
      961 cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt catatggctt
     1021 tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac aaagctaagg
     1081 agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag attttaagcc
     1141 atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat tctggattgc
     1201 ctgacaatgc cagacgataa gcggccgcga ctctagatca taatcagcca taccacattg
     1261 tagaagttgc cc
//
