LOCUS       sequence-85214-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VEL-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..104
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             114..794
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattccc accatgggtg
       61 tcgcagattt gatcaagaaa ttcgaaagca tctcaaagga agaacgggat ccaatgagtg
      121 cgattaagcc agacatgaag atcaaactcc gtatggaagg caacgtaaac gggcaccact
      181 ttgtgatcga cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg
      241 aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact gcattcctgt
      301 acggcaacag ggtattcgcc aaatatccag acaacataca agactatttt aagcagtcgt
      361 ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaacg
      421 ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta
      481 ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca
      541 ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tgtagagatg gctttgttgc
      601 ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg
      661 gtgtcaagtt accaggcgcc cactttgtgg accactgcat tgagatttta agccatgaca
      721 aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca
      781 atgccagacg ataagcggcc gcgactctag atcataatca gccataccac atttgtagag
      841 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      901 gcaattgttg ttgttaactt gtt
//
