LOCUS       sequence-85221-e        1288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 588, Emission:
            635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, 
            Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, 
            Chudakov DM
  TITLE     Bright far-red fluorescent protein for whole-body imaging.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):741-6.
  PUBMED    17721542
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):741-6."
COMMENT     Sequence Label: mKate-pBAD
FEATURES             Location/Qualifiers
     source          1..1288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             339..361
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             381..398
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             402..434
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             447..467
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             474..1187
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(1233..1250)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ctgcggattc cgcgacagat gtaatcttgg ctcacatctt actccgcatt ggacatgtcc
       61 caattggcat acgactgcgt catgctttaa ctgtctttcc cgctaacaac ggtaaccggt
      121 attaaagcat tcgtaacaag cggaacaaag gccatgacaa aaacgcgtaa caaagtgtct
      181 tataatcacg ccagaaaagt cccccattga ttatttgcac ggcgtcacac tttgctatgc
      241 caatagcatt ttatccataa gattagcgat actacctgac gctttttatc gcaactctct
      301 actgtttctc catacccgtt ttttgggcta gaaataattt tgtttaactt taagaaggag
      361 atatacatat gcggggttct catcatcatc atcatcatgg tatggctagc atgactggtg
      421 gacagcaaat gggtcgggat ctgtacgaga acctgtactt ccagggctcg agcatggtgt
      481 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
      541 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
      601 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
      661 ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc atccccgact
      721 tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
      781 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
      841 tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
      901 gctgggaggc ctccaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaagcg
      961 acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag accacataca
     1021 gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg gacagaagac
     1081 tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg
     1141 ccagatactg cgacctccct agcaaactgg ggcacaaact taattgagaa ttcgaagctt
     1201 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
     1261 agcgtctata ctagaagcta cgtaaggg
//
