LOCUS       sequence-85260-e        3550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 568, 
            Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3550)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    2560..2571
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      361 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
      541 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctggca gcggtggagg cttggaggag
      781 ccggaggccg cgacgcggac agccgcggcc gtggattgca aagacagacc cgggttccca
      841 gtgaagaggt taatacaagc ccgcttgccc ttcaaacgcc tgaaccttgt tcccaaggag
      901 aaagttgagg aagacacatc accaaaagca gctgtggaaa gcaaagtccc ggatctacaa
      961 ttgtctttgg gcacttttga aagccagtgt cacacaggct ctcacgtagg cttgagtaca
     1021 aagttggtcg gtggccaggg ccccattgat agcttcttga gggcaacaat caagccagta
     1081 ccgagtgtgg tcattatcga cctgacggag aactgcagtg acattccaga cagccctgag
     1141 ggccacagtg aactaagtcc tgacactgct ggtgtggtca ccacagtgga aggagctgcc
     1201 aagcagcagg agcactctgc agcagagctg tgtctcctag agaccccttc agacatcact
     1261 tgtcacatgg aagaggagcc tgggagtcca ggagacccaa agaggacggg tgactgtcag
     1321 gctggttcac tacagagctg tccggagctg accccaggtt ctaggacatg ccccaccaag
     1381 gagttatcaa gctggagcaa ggcaggggat ctcctattta tagagaaagt gcctgtggtg
     1441 gtgctagagg acatcctggc cactaagccc tccattgcct ctcttcccat gatgtccctg
     1501 gataggagtg tcacttcaga gagtgaaata ctggagtcct gccccgagga tgactccata
     1561 ctgagccact cctccaccaa ctcctcttct ccaaccagct ctcctgaggg accatccaca
     1621 cccccagaac atcggggtgg gagaagctcc ccctccaccc cagcctgcag agttgccaag
     1681 aactttgtca aaggctccac agagaagggc aggagcaaac tgcacagaga tagagaacag
     1741 cagagggaag agaaggaaaa gctgagggaa gagatacgac gagccaagga ggaggcacgg
     1801 aagaagaagg aggaggagaa ggagctgaag gaaaaggagc ggagggaaaa gcgggagaaa
     1861 gatgagaagg agaaggcgga gaagcagcgg ctaaaggagg aaaaacgcaa ggagcggcag
     1921 gaagctctgg aggctaaact ggaggagaag aggaagaagg aggaagagaa gcggttacgg
     1981 gaggaagaga agcggttacg ggaagaagag aagcgcatta aggcagaaaa ggcagagatc
     2041 actcgattct tccagaaacc gaagactccg caggctccca agaccctggc cggttcctgt
     2101 ggtaagtttg ccccctttga aatcaaagaa cacatggtgc tggctccacg gtgccgagct
     2161 gccttggatc aagacctgtg cgatcagctg gaccagctcc tgcagcagca gagtgtggca
     2221 agcaccttcc tcagtgacct aaaaagccgg ctgcccttga gatcggggcc cacccgggtc
     2281 tgtggccatg acacagacat catgaacagg gatgtggtca ttgtggagag cagcaaggtg
     2341 gacggtgtgt ctgaaaggaa gaagtttggc cgcatgaagc ttctgcagtt ctcagagaac
     2401 caccggccag catactgggg gacgtggaac aagaagacag ccatcatccg cccccggaac
     2461 ccctgggccc aggacaaaga ccttcttgac tatgaggtag acagtgacga tgagtgggaa
     2521 gaggaggaac ctggggagtc tttgtcccac agtgaggggg atgaggatga cgatgtgggg
     2581 gaagatgagg atgaggacga tggcttcttt gtgccccatg ggtacttatc tgaagatgaa
     2641 ggcgtgactg aggagtgtgc agaccctgag aaccacaaag tacaccagaa gctgaaggcc
     2701 aaggagtggg atgagctcct agccaagggc aagcgctttc gtgtgctgca gcctgtgcac
     2761 gtgggatgtg tatgggcatc agaggcagcc aactgcacga gttccgactt gaagctgctg
     2821 cagcagttca ctgcatgcct gctggatgtg gcatcccctg atgagccaga acccggggcc
     2881 tccaggaggg aaaagaggga ccagcatatc ctggcccagt tgcttccgct gctacatggc
     2941 aatgtgaatg ggagcaaggt gatcatccat gaattccagg agcaatgccg caggggcctg
     3001 ctgacccttc caagtcccac cccacacctg cagatgccca acttggagga tgctgttgct
     3061 gtgccatcca aggccaggct gaagcgcctt atctctgaga actctgccta tgagaagcgg
     3121 cccaacttcc gtatgtgctg gtatgtgcac ccagaggtgc tcaagagctt tggccaggag
     3181 tgcctgccag tgccctgcca gtggacctac atcaccacca tgccctcagc acccagggaa
     3241 gacagtggca gtgcttctac tgaggggcct ggccagagca cacctatgct actcaagcgg
     3301 aagccagcgg ccaccatgtg catcacgcag tttatgaaga agcggcgcta tgatggacag
     3361 gttgggtctg gggacatgga tggcttccag gcagacacag aagaggatga agaggatgac
     3421 acagactgta tgatcataga tgtgccagat gttgggagtg acgtgtcaga agctcccata
     3481 cctgctccca cactttgcaa gtgaggatcc accggatcta gataactgat cataatcagc
     3541 cataccacat
//
