LOCUS       sequence-85267-e        1349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1349)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    531..583
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      591..600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             597..1307
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1010..1029
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgacgtcaga tccgctagcc accatgggcc ccaagcggcg gcagctgacg ttccgggaga
       61 agtcgcggat catccaggag gtggaggaga acccggacct acgcaagggc gagatcgcgc
      121 ggcgcttcaa catcccgccg tccacgctga gcacgatcct gaagaacaag cgcgccatcc
      181 tggcgtcgga gcgcaagtac ggggtggcct ccacctgccg caagaccaac aagctgtctc
      241 cctacgacaa gctcgagggc ttgctcatcg cctggttcca gcagatccgc gccgccggcc
      301 tgccggtcaa gggcatcatc ctcaaggaga aggcgctgcg catagccgag gagctgggca
      361 tggacgactt caccgcctcc aacggctggc tggaccgctt ccgccggcgc cacggcgtgg
      421 tgtcctgcag cggcgtggcc cgcgcccgcg cgcgaaacgc tgccccccgc accccggcgg
      481 cgcctgccag tccggccgcg gtgccctcgg agggcagtgg cgggagcact ctcgagctca
      541 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg
      601 tgagcaaggg cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      661 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      721 cctacgaggc ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg
      781 cctgggacat cctgtcccct cagttcatgt acggctccaa ggtctacatt aagcacccag
      841 ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga
      901 tgaacttcga ggacggcggc attattcacg ttaaccagga ctcctccctg caggacggcg
      961 tgttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     1021 agaagaagac catgggctgg gaggcctccg aggagcggat gtaccccgag gacggcgccc
     1081 tgaagagcga gatcaagaag aggctgaagc tgaaggacgg cggccactac gccgccgagg
     1141 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca
     1201 tcaagttgga catcgtgtcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     1261 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc
     1321 tagatcataa tcagccatac cacattgag
//
