LOCUS       sequence-85274-e        1531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1531)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Dectin1A-N-10
FEATURES             Location/Qualifiers
     source          1..1531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             790..1500
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1203..1222
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agatccgcta gcgccaccat ggaatatcat cctgatttag aaaatttgga tgaagatgga
       61 tatactcaat tacacttcga ctctcaaagc aataccagga tagctgttgt ttcagagaaa
      121 ggatcgtgtg ctgcatctcc tccttggcgc ctcattgctg taattttggg aatcctatgc
      181 ttggtaatac tggtgatagc tgtggtcctg ggtaccatgg ctatttggag atccaattca
      241 ggaagcaaca cattggagaa tggctacttt ctatcaagaa ataaagagaa ccacagtcaa
      301 cccacacaat catctttaga agacagtgtg actcctacca aagctgtcaa aaccacaggg
      361 gttctttcca gcccttgtcc tcctaattgg attatatatg agaagagctg ttatctattc
      421 agcatgtcac taaattcctg ggatggaagt aaaagacaat gctggcaact gggctctaat
      481 ctcctaaaga tagacagctc aaatgaattg ggatttatag taaaacaagt gtcttcccaa
      541 cctgataatt cattttggat aggcctttct cggccccaga ctgaagtacc atggctctgg
      601 gaggatggat caacattctc ttctaactta tttcagatca gaaccacagc tacccaagaa
      661 aacccatctc caaattgtgt atggattcac gtgtcagtca tttatgacca actgtgtagt
      721 gtgccctcat atagtatttg tgagaagaag ttttcaatga gctccggagg ggatccaccg
      781 gtcgccacca tggtgagcaa gggcgaggag aataacatgg ccatcatcaa ggagttcatg
      841 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      901 ggcgagggcc gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc
      961 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggtctac
     1021 attaagcacc cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg
     1081 tgggagcgcg tgatgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc
     1141 ctgcaggacg gcgtgttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1201 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccgaggagcg gatgtacccc
     1261 gaggacggcg ccctgaagag cgagatcaag aagaggctga agctgaagga cggcggccac
     1321 tacgccgccg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1381 tacatcgtcg acatcaagtt ggacatcgtg tcccacaacg aggactacac catcgtggaa
     1441 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     1501 gcggccgcga ctctagatca taattcagcc a
//
