LOCUS       sequence-85276-e        3493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cadherin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3493)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Desmocollin1-N-18
FEATURES             Location/Qualifiers
     source          1..3493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2748..2757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2754..3464
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3167..3186
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgccaccatg gctctggcct ctgctgcccc agggagcatc ttctgtaagc
       61 agctcctttt ctctctcctg gttttaacat tactttgcga tgcttgtcag aaagtttatc
      121 ttcgagttcc ttctcatctt caggctgaaa cacttgtagg caaagtgaat ctggaggagt
      181 gtctcaagtc ggccagccta atccggtcca gtgaccctgc cttcaggatt ctagaagatg
      241 gctcaattta cacaacacat gacctcattt tgtcttctga aaggaaaagt ttttccattt
      301 tcctttcaga tggtcagaga cgggaacaac aagagataaa agttgtactg tcagcaagag
      361 aaaacaagtc tcctaagaag agacatacca aagacacagc cctcaagcgc agcaagagac
      421 gatgggctcc tattccagct tcattgatgg agaactcgtt gggtccattt ccacaacacg
      481 ttcagcagat ccaatctgat gctgcacaga attacaccat cttttattcc ataagtgggc
      541 caggcgtgga caaagaaccc ttcaatttgt tttacataga gaaagacact ggggatatct
      601 tttgtacaag gagcattgac cgtgagaaat acgaacagtt tgcgttatat ggctatgcaa
      661 caactgcaga tggctatgca ccagaatatc cactcccttt gatcatcaaa attgaagatg
      721 ataatgataa cgccccatat tttgaacaca gagtgactat ctttactgtg cctgaaaatt
      781 gccgatccgg cacttcagtg ggaaaagtga ccgccacaga ccttgacgaa cctgacactc
      841 tccatactcg tctgaaatat aaaatcttac aacaaatccc agatcatcca aagcatttct
      901 ccatacaccc agataccgga gtcatcacca caactacacc ttttctggat agagaaaaat
      961 gtgatactta ccagttaata atggaagtgc gagacatggg tggtcagcct ttcggtttat
     1021 ttaatacagg aacaattact atttcacttg aggatgaaaa tgacaatcca ccatctttca
     1081 cagaaacttc ttatgttaca gaagtagaag aaaacagaat tgacgtggag attttgcgaa
     1141 tgaaggtaca ggatcaggat ttgccaaaca ctcctcactc aaaggctgta tacaaaatct
     1201 tacaaggaaa tgaaaatgga aacttcataa ttagcacaga tccaaataca aatgaaggag
     1261 tgctgtgtgt tgtcaagcca ttgaactatg aagtcaatcg ccaagttatt ttgcaagttg
     1321 gtgtcattaa cgaggcacaa ttctctaaag cagcgagctc acaaactcct acaatgtgca
     1381 ctacaactgt caccgttaaa attatagaca gtgatgaggg ccctgaatgc caccctccag
     1441 tgaaagttat tcagagtcaa gatggcttcc cagctggcca agaactcctt ggatacaaag
     1501 cactggaccc ggaaatatcc agtggtgaag gcttaaggta tcagaagtta ggggatgaag
     1561 ataactggtt tgaaattaat caacacactg gcgacttgag aactctaaaa gtactagata
     1621 gagaatccaa atttgtaaaa aacaaccaat acaatatttc agttgttgca gtggatgcag
     1681 ttggccgatc ttgcactgga acattagtag ttcatttgga tgattacaac gatcacgcac
     1741 ctcaaattga caaagaagtg accatttgtc agaataatga ggattttgct gttctgaaac
     1801 ctgtagatcc agatggacct gaaaatggac caccttttca attctttctg gataattctg
     1861 ccagtaaaaa ctggaacata gaagaaaagg atggtaaaac tgccattctt cgtcaacggc
     1921 aaaatcttga ttataactat tattctgtgc ctattcaaat aaaagacagg catggtttag
     1981 ttgcaacaca tatgttaaca gtgagagtat gtgactgttc aactccatct gagtgtagaa
     2041 tgaaggataa aagtacaaga gacgttagac caaatgtaat acttggaaga tgggctattc
     2101 ttgctatggt gttgggttct gtattgttat tatgtattct gtttacatgt ttctgtgtca
     2161 ctgctaagag aacagtcaag aaatgttttc cagaagacat agcccagcaa aatttaattg
     2221 tatcaaatac tgaaggacct ggagaagaag taacggaagc aaatattaga ctccccatgc
     2281 agacatccaa catttgtgac acaagcatgt ctgttggtac tgttggtggc cagggaatca
     2341 aaacacagca aagttttgag atggtcaaag gaggctacac tttggattcc aacaaaggag
     2401 gtggacatca gaccttggag tccgtcaagg gagtggggca gggagatact ggcagatatg
     2461 cgtacacgga ctggcagagt ttcacccaac ctcggcttgg cgaaaaggtg tatttgtgtg
     2521 gacaagatga ggagcataaa cattgtgaag actacgtttg ttcgtataac tatgaaggca
     2581 aaggttctct ggccggctca gtaggttgct gcagcgatcg gcaggaagaa gagggactgg
     2641 agtttctaga tcacctggaa cccaaattta ggacattagc aaagacatgc atcaagaaaa
     2701 gcagcggtgg agcaagcgca gccagtggta gcgcggatcc accggtcgcc accatggtga
     2761 gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     2821 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     2881 acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     2941 gggacatcct gtcccctcag ttcatgtacg gctccaaggt ctacattaag cacccagccg
     3001 acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga
     3061 acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtgt
     3121 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     3181 agaagaccat gggctgggag gcctccgagg agcggatgta ccccgaggac ggcgccctga
     3241 agagcgagat caagaagagg ctgaagctga aggacggcgg ccactacgcc gccgaggtca
     3301 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca
     3361 agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     3421 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgactctag
     3481 atcataatca gcc
//
