LOCUS       sequence-85278-e        3956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3956)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Desmoglein1-N-18
FEATURES             Location/Qualifiers
     source          1..3956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3213..3222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3219..3929
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3632..3651
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgctagcgt cgccaccatg gactggagtt tcttcagagt agttgcagtg ctgttcattt
       61 ttctggtggt ggtagaagtt aacagtgagt tccgaatcca ggtaagagat tataacacta
      121 aaaatggcac catcaaatgg cattcaatcc gaaggcagaa acgtgaatgg atcaagttcg
      181 cagcagcctg tcgtgaaggt gaagacaact caaagaggaa cccaatcgcc aaaattcact
      241 cagattgtgc tgcaaaccag caagttacat accgcatctc tggagtagga attgatcagc
      301 caccatatgg gatctttgtc attaatcaga aaactggtga aattaatata acatccatag
      361 ttgatcgaga ggtcactcct ttcttcatta tctactgccg agctctgaac tcaatgggcc
      421 aagatttaga gaggcctcta gagctcagag tcagggtttt ggatataaat gacaaccctc
      481 cagtgttttc aatggctaca tttgcaggac aaatagaaga aaattctaat gcaaatacac
      541 tggtgatgat actcaatgct actgacgcag atgaaccgaa caatttgaac tcaaaaatag
      601 ccttcaagat tataagacaa gaaccttcag attcaccaat gtttattatc aacagaaata
      661 ctggagaaat tcgaacgatg aataattttc tagacagaga gcaatacggc cagtatgctc
      721 ttgctgtaag aggctctgac cgagatggtg gggcagatgg catgtcagcg gaatgtgagt
      781 gcaacattaa aatcctcgat gtcaatgata atatccctta catggaacag tcttcatata
      841 ccatagaaat tcaagaaaat actctaaatt caaatttgct cgaaattaga gtaattgatt
      901 tggatgaaga gttctcagct aactggatgg cagtaatttt ctttatctct ggaaatgaag
      961 gaaattggtt tgagatagaa atgaatgaaa gaacaaatgt gggaatttta aaggttgtta
     1021 agcccttaga ttatgaagct atgcagagtc tgcaactcag tattggtgtc agaaataaag
     1081 ctgaatttca tcattcaatt atgtctcaat ataaactgaa agcatctgca atttctgtga
     1141 ctgtgttaaa tgtaattgaa ggcccagtgt ttcgtccagg ttcaaagaca tatgttgtaa
     1201 ctggtaatat gggatcaaat gataaagtgg gagactttgt agctactgac ctggacacag
     1261 gtagaccttc aacgactgtt aggtatgtaa tgggaaataa tccagctgac ctgcttgctg
     1321 ttgattcaag aacaggcaaa ctcactttga aaaataaagt taccaaggaa cagtacaata
     1381 tgctcggagg aaaataccaa ggaacgattc tctctataga tgataatctt caaagaactt
     1441 gcactggtac aattaatatt aacattcaaa gttttggtaa tgacgacagg actaatacag
     1501 agccgaacac taaaattact accaatactg gcagacaaga aagtacttct tccactaact
     1561 atgataccag cacaacttct actgactcta gccaagtata ttcttctgaa cccggaaacg
     1621 gagccaaaga tttgttatca gacaatgtac attttggtcc tgctggcatt ggactcctca
     1681 tcatgggatt cttggtctta ggattggtcc catttttgat gatctgttgt gattgtggag
     1741 gtgctcctcg tagtgcagct ggctttgagc ctgttcccga atgttcagat ggagcaattc
     1801 attcatgggc agtagaagga ccacagcctg aacccaggga tataaccact gtcataccac
     1861 aaataccacc tgataacgca aatataattg aatgcattga caactcagga gtttatacaa
     1921 atgagtatgg tggcagagaa atgcaagacc tgggaggagg agagagaatg acaggatttg
     1981 aactaacaga gggagttaaa acttcaggaa tgcctgagat atgtcaagaa tactctggaa
     2041 cattaagaag aaattctatg agggaatgta gagaaggagg tctgaatatg aatttcatgg
     2101 aaagctactt ctgtcagaaa gcatatgctt acgcagatga agatgaagga cgcccatcta
     2161 atgactgttt gctcatatat gacatcgaag gtgtaggttc ccctgctggc tctgtgggtt
     2221 gttgtagctt cattggagaa gacctggatg acagcttctt ggataccctg ggacctaaat
     2281 ttaagaagtt ggcagacatc agcctaggaa aagaatcata tccagacctt gatccttctt
     2341 ggccaccaca aagcactgaa ccagtttgcc ttcctcagga aacagagccc gttgttagtg
     2401 gacacccacc aatctcccca catttcggca ctaccacagt aatttctgag agcacctatc
     2461 cctcgggacc tggtgtactg catcctaagc ctattctcga tcctctgggc tatggtaatg
     2521 tcactgtgac cgagtcttac accacctctg acactctgaa gccctctgtg cacgttcacg
     2581 ataaccgacc agcatcaaac gtggtagtga cagagagagt ggtcggccca atctctggcg
     2641 ctgatttgca tggaatgtta gagatgcctg acttgcgaga tgggtcgaat gttatagtga
     2701 cagaaagggt aatagcacca agctctagtc tacccacctc tctgactatc catcatccta
     2761 gagagtcttc aaatgtggta gtgacagaaa gagtaatcca accaacttcc ggcatgatag
     2821 gtagtctgag tatgcacccc gagttagcca atgcccacaa tgtcattgtg acagagaggg
     2881 ttgtttctgg tgctggcgta actggaatta gtggcaccac tgggatcagc ggtggcatag
     2941 gcagcagtgg cctggttggc accagcatgg gtgctgggag cggtgccctg agtggagctg
     3001 gcataagtgg tggtggcatt ggcctgagca gcttgggagg gacagccagc attggccaca
     3061 tgaggagttc ctctgaccat cactttaacc aaaccattgg gtccgcctcc cctagcacag
     3121 ctcgaagtcg aatcacaaag tatagtaccg tgcaatatag caagagcagc ggtggagcaa
     3181 gcgcagccag tggtagcgcg gatccaccgg tcgccaccat ggtgagcaag ggcgaggaga
     3241 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     3301 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga
     3361 ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     3421 ctcagttcat gtacggctcc aaggtctaca ttaagcaccc agccgacatc cccgactact
     3481 tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg
     3541 gcattattca cgttaaccag gactcctccc tgcaggacgg cgtgttcatc tacaaggtga
     3601 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     3661 gggaggcctc cgaggagcgg atgtaccccg aggacggcgc cctgaagagc gagatcaaga
     3721 agaggctgaa gctgaaggac ggcggccact acgccgccga ggtcaagacc acctacaagg
     3781 ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg gacatcgtgt
     3841 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     3901 ccggcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcag
//
