LOCUS       sequence-85282-e        1675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 568, Emission: 
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1675)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-EB3-7
FEATURES             Location/Qualifiers
     source          1..1675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      920..929
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             926..1636
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1339..1358
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt cgactgccac
       61 catggccgtc aatgtgtact ccacatctgt gaccagtgaa aatctgagtc gccatgatat
      121 gcttgcatgg gtcaacgact ccctgcacct caactatacc aagatagaac agctttgttc
      181 aggggcagcc tactgccagt tcatggacat gctcttcccc ggctgtgtgc acttgaggaa
      241 agtgaagttc caggccaaac tagagcatga atacatccac aacttcaagg tgctgcaagc
      301 agctttcaag aagatgggtg ttgacaaaat cattcctgta gagaaattag tgaaaggaaa
      361 attccaagat aattttgagt ttattcagtg gtttaagaaa ttctttgacg caaactatga
      421 tggaaaggat tacaaccctc tgctggcgcg gcagggccag gacgtagcgc cacctcctaa
      481 cccaggtgat cagatcttca acaaatccaa gaaactcatt ggcacagcag ttccacagag
      541 gacgtccccc acaggcccaa aaaacatgca gacctctggc cggctgagca atgtggcccc
      601 cccctgcatt ctccggaaga atcctccatc agcccgaaat ggcggccatg agactgatgc
      661 ccaaattctt gaactcaacc aacagctggt ggacttgaag ctgacagtgg atgggctgga
      721 gaaggaacgt gacttctact tcagcaaact tcgtgacatc gagctcatct gccaggagca
      781 tgaaagtgaa aacagccctg ttatctcagg catcattggc atcctctatg ccacagagga
      841 aggattcgca ccccctgagg acgatgagat tgaagagcat caacaagaag accaggacga
      901 gtacctggat ccaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccat
      961 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     1021 cgagatcgag ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa
     1081 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     1141 cggctccaag gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt
     1201 ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt
     1261 taaccaggac tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac
     1321 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga
     1381 ggagcggatg taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct
     1441 gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt
     1501 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga
     1561 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     1621 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatt
//
