LOCUS       sequence-85289-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..63
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     14..34
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..815
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     521..540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
       61 gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg
      121 gcgaggagaa taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
      181 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      241 cctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      301 tcctgtcccc tcagttcatg tacggctcca aggtctacat taagcaccca gccgacatcc
      361 ccgactactt caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg
      421 aggacggcgg cattattcac gttaaccagg actcctccct gcaggacggc gtgttcatct
      481 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      541 ccatgggctg ggaggcctcc gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg
      601 agatcaagaa gaggctgaag ctgaaggacg gcggccacta cgccgccgag gtcaagacca
      661 cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg
      721 acatcgtgtc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
      781 gccactccac cggcggcatg gacgagctgt acaagtccgg actcagatct aagctgaacc
      841 ctcctgatga gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc tgagaattct
      901 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
      961 cataccacat ttgtagaggt tttacttgct ttaa
//
