LOCUS       sequence-85294-e        2405 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2405)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-FYN2-N-10
FEATURES             Location/Qualifiers
     source          1..2405
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1656..1665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1662..2372
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2075..2094
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgccacc atgggctgtg tgcaatgtaa ggataaagaa gcaacaaaac
       61 tgacggagga gagggacggc agcctgaacc agagctctgg gtaccgctat ggcacagacc
      121 ccacccctca gcactacccc agcttcggtg tgacctccat ccccaactac aacaacttcc
      181 acgcagccgg gggccaagga ctcaccgtct ttggaggtgt gaactcttcg tctcatacgg
      241 ggaccttgcg tacgagagga ggaacaggag tgacactctt tgtggccctt tatgactatg
      301 aagcacggac agaagatgac ctgagttttc acaaaggaga aaaatttcaa atattgaaca
      361 gctcggaagg agattggtgg gaagcccgct ccttgacaac tggagagaca ggttacattc
      421 ccagcaatta tgtggctcca gttgactcta tccaggcaga agagtggtac tttggaaaac
      481 ttggccgaaa agatgctgag cgacagctat tgtcctttgg aaacccaaga ggtacctttc
      541 ttatccgcga gagtgaaacc accaaaggtg cctattcact ttctatccgt gattgggatg
      601 atatgaaagg agaccatgtc aaacattata aaattcgcaa acttgacaat ggtggatact
      661 acattaccac ccgggcccag tttgaaacac ttcagcagct tgtacaacat tactcagaga
      721 gagctgcagg tctctgctgc cgcctagtag ttccctgtca caaagggatg ccaaggctta
      781 ccgatctgtc tgtcaaaacc aaagatgtct gggaaatccc tcgagaatcc ctgcagttga
      841 tcaagagact gggaaatggg cagtttgggg aagtatggat gggtacctgg aatggaaaca
      901 caaaagtagc cataaagact cttaaaccag gcacaatgtc ccccgaatca ttccttgagg
      961 aagcgcagat catgaagaag ctgaagcacg acaagctggt ccagctctat gcagtggtgt
     1021 ctgaggagcc catctacatc gtcaccgagt atatgaacaa aggaagttta ctggatttct
     1081 taaaagatgg agaaggaaga gctctgaaat taccaaatct tgtggacatg gcagcacagg
     1141 tggctgcagg aatggcttac atcgagcgca tgaattatat ccatagagat ctgcgatcag
     1201 caaacattct agtggggaat ggactcatat gcaagattgc tgacttcgga ttggcccgat
     1261 tgatagaaga caatgagtac acagcaagac aaggtgcaaa gttccccatc aagtggacgg
     1321 cccccgaggc agccctgtac gggaggttca caatcaagtc tgacgtgtgg tcttttggaa
     1381 tcttactcac agagctggtc accaaaggaa gagtgccata cccaggcatg aacaaccggg
     1441 aggtgctgga gcaggtggag cgaggctaca ggatgccctg cccgcaggac tgccccatct
     1501 ctctgcatga gctcatgatc cactgctgga aaaaggaccc tgaagaacgc cccacttttg
     1561 agtacttgca gagcttcctg gaagactact ttaccgcgac agagccccag taccaacctg
     1621 gtgaaaacct ggcatccagc ggggatccac cggtcgccac catggtgagc aagggcgagg
     1681 agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1741 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gaggcctttc
     1801 agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1861 cccctcagtt catgtacggc tccaaggtct acattaagca cccagccgac atccccgact
     1921 acttcaagct gtccttcccc gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg
     1981 gcggcattat tcacgttaac caggactcct ccctgcagga cggcgtgttc atctacaagg
     2041 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     2101 gctgggaggc ctccgaggag cggatgtacc ccgaggacgg cgccctgaag agcgagatca
     2161 agaagaggct gaagctgaag gacggcggcc actacgccgc cgaggtcaag accacctaca
     2221 aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag ttggacatcg
     2281 tgtcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     2341 ccaccggcgg catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc
     2401 catac
//
