LOCUS       sequence-85302-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Adaptor, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Homer1-C-18
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..732
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     438..457
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc
       61 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      121 gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag
      181 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      241 ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc
      301 cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat tattcacgtt
      361 aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct gcgcggcacc
      421 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccgag
      481 gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag gctgaagctg
      541 aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa gaagcccgtg
      601 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca caacgaggac
      661 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      721 gagctgtaca agtccggagg gagcggcagt gggtccagtg gagggagcag tggaagcgga
      781 gggagcgggg aacaacctat cttcagcact cgagctcatg tcttccagat cgacccaaac
      841 acaaagaaga actgggtacc caccagcaag catgcagtta ctgtgtctta tttctatgac
      901 agcacaagga atgtgtatag gataatcagt ttagacggct caaaggcaat aataaatagc
      961 accatcactc caaacatgac atttactaaa acatctcaaa agtttggcca atgggctgat
     1021 agccgggcaa acactgttta tggactggga ttctcctctg agcatcatct ctcaaaattt
     1081 gcagaaaagt ttcaggaatt taaagaagct gctcggctgg caaaggagaa gtcgcaggag
     1141 aagatggaac tgaccagtac cccttcacag gaatcagcag gaggagatct tcagtctcct
     1201 ttaacaccag aaagtatcaa tgggacagat gatgagagaa cacccgatgt gacacagaac
     1261 tcagagccaa gggctgagcc agctcagaat gcattgccat tttcacatag tgccggggat
     1321 cgaacccagg gcctctctca tgctagttca gccatcagca aacactggga ggctgaacta
     1381 gccacgctca aggggaacaa tgccaagctc accgcagcgc tgctggagtc cactgccaac
     1441 gtgaagcagt ggaagcaaca gctggctgcc taccaggagg aggcagagcg gctgcacaag
     1501 cgggtcacgg agctggaatg tgttagtagt caagcaaacg cggtgcacag ccacaagaca
     1561 gagctgagtc agacagtgca ggagctggaa gagaccctaa aagtaaagga agaggaaata
     1621 gaaagattaa aacaagaaat tgataacgcc agagaacttc aagaacagag ggactctttg
     1681 actcagaaac tacaggaagt tgagattcga aataaagacc tggaggggca gctgtcggag
     1741 ctggagcagc gcctggagaa gagccagagc gagcaggacg ctttccgcag taacctgaag
     1801 actctcctag agattctgga cgggaaaata tttgaactaa cagaattgcg ggataatttg
     1861 gccaagctac tagaatgcag ctaaggatcc accggatcta gataactgat cataatc
//
