LOCUS       sequence-85304-e        3232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 568, Emission: 
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3232)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Integrin-Beta1-N-18
FEATURES             Location/Qualifiers
     source          1..3232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2481..2490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2487..3197
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2900..2919
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agaccgccat gaatttacaa ccaattttct
       61 ggattggact gatcagttca gtttgctgtg tgtttgctca aacagatgaa aatagatgtt
      121 taaaagcaaa tgccaaatca tgtggagaat gtatacaagc agggccaaat tgtgggtggt
      181 gcacaaattc aacattttta caggaaggaa tgcctacttc tgcacgatgt gatgatttag
      241 aagccttaaa aaagaagggt tgccctccag atgacataga aaatcccaga ggctccaaag
      301 atataaagaa aaataaaaat gtaaccaacc gtagcaaagg aacagcagag aagctcaagc
      361 cagaggatat tactcagatc caaccacagc agttggtttt gcgattaaga tcaggggagc
      421 cacagacatt tacattaaaa ttcaagagag ctgaagacta tcccattgac ctctactacc
      481 ttatggacct gtcttactca atgaaagacg atttggagaa tgtaaaaagt cttggaacag
      541 atctgatgaa tgaaatgagg aggattactt cggacttcag aattggattt ggctcatttg
      601 tggaaaagac tgtgatgcct tacattagca caacaccagc taagctcagg aacccttgca
      661 caagtgaaca gaactgcacc agcccattta gctacaaaaa tgtgctcagt cttactaata
      721 aaggagaagt atttaatgaa cttgttggaa aacagcgcat atctggaaat ttggattctc
      781 cagaaggtgg tttcgatgcc atcatgcaag ttgcagtttg tggatcactg attggctgga
      841 ggaatgttac acggctgctg gtgttttcca cagatgccgg gtttcacttt gctggagatg
      901 ggaaacttgg tggcattgtt ttaccaaatg atggacaatg tcacctggaa aataatatgt
      961 acacaatgag ccattattat gattatcctt ctattgctca ccttgtccag aaactgagtg
     1021 aaaataatat tcagacaatt tttgcagtta ctgaagaatt tcagcctgtt tacaaggagc
     1081 tgaaaaactt gatccctaag tcagcagtag gaacattatc tgcaaattct agcaatgtaa
     1141 ttcagttgat cattgatgca tacaattccc tttcctcaga agtcattttg gaaaacggca
     1201 aattgtcaga aggagtaaca ataagttaca aatcttactg caagaacggg gtgaatggaa
     1261 caggggaaaa tggaagaaaa tgttccaata tttccattgg agatgaggtt caatttgaaa
     1321 ttagcataac ttcaaataag tgtccaaaaa aggattctga cagctttaaa attaggcctc
     1381 tgggctttac ggaggaagta gaggttattc ttcagtacat ctgtgaatgt gaatgccaaa
     1441 gcgaaggcat ccctgaaagt cccaagtgtc atgaaggaaa tgggacattt gagtgtggcg
     1501 cgtgcaggtg caatgaaggg cgtgttggta gacattgtga atgcagcaca gatgaagtta
     1561 acagtgaaga catggatgct tactgcagga aagaaaacag ttcagaaatc tgcagtaaca
     1621 atggagagtg cgtctgcgga cagtgtgttt gtaggaagag ggataataca aatgaaattt
     1681 attctggcaa attctgcgag tgtgataatt tcaactgtga tagatccaat ggcttaattt
     1741 gtggaggaaa tggtgtttgc aagtgtcgtg tgtgtgagtg caaccccaac tacactggca
     1801 gtgcatgtga ctgttctttg gatactagta cttgtgaagc cagcaacgga cagatctgca
     1861 atggccgggg catctgcgag tgtggtgtct gtaagtgtac agatccgaag tttcaagggc
     1921 aaacgtgtga gatgtgtcag acctgccttg gtgtctgtgc tgagcataaa gaatgtgttc
     1981 agtgcagagc cttcaataaa ggagaaaaga aagacacatg cacacaggaa tgttcctatt
     2041 ttaacattac caaggtagaa agtcgggaca aattacccca gccggtccaa cctgatcctg
     2101 tgtcccattg taaggagaag gatgttgacg actgttggtt ctattttacg tattcagtga
     2161 atgggaacaa cgaggtcatg gttcatgttg tggagaatcc agagtgtccc actggtccag
     2221 acatcattcc aattgtagct ggtgtggttg ctggaattgt tcttattggc cttgcattac
     2281 tgctgatatg gaagctttta atgataattc atgacagaag ggagtttgct aaatttgaaa
     2341 aggagaaaat gaatgccaaa tgggacacgg gtgaaaatcc tatttataag agtgccgtaa
     2401 caactgtggt caatccgaag tatgagggaa aaggtagcgc atccgggggt gcgtcaggcg
     2461 gcagcggatc tggaccggtc gccaccatgg tgagcaaggg cgaggagaat aacatggcca
     2521 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     2581 tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga
     2641 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     2701 acggctccaa ggtctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct
     2761 tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg
     2821 ttaaccagga ctcctccctg caggacggcg tgttcatcta caaggtgaag ctgcgcggca
     2881 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctccg
     2941 aggagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag aggctgaagc
     3001 tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg
     3061 tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg
     3121 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     3181 acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac ca
//
