LOCUS       sequence-85308-e        1723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1723)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-LAMP1-phLuorin-N-8
FEATURES             Location/Qualifiers
     source          1..1723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..133
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             134..844
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(161..189)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     784..804
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             989..1699
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1402..1421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagcgcc accatggaca gcaaaggttc
       61 gtcgcagaaa gggtcccgcc tgctcctgct gctggtggtg tcaaatctac tcttgtgcca
      121 gggtgtggtc tccagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      181 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      241 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      301 gccaacactt gtcactactt taacttatgg tgttcaatgc ttttcaagat acccagatca
      361 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      421 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      481 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      541 tggacacaaa ttggaataca actataacga tcaccaggtg tacatcatgg cagacaaaca
      601 aaagaatgga atcaaagcta acttcaaaat tagacacaac attgaagatg gaggcgttca
      661 actagcagac cattatcaac aaaatactcc aattggcgat gggcccgtcc ttttaccaga
      721 caaccattac ctgtttacaa cttctactct ttcgaaagat cccaacgaaa agagagacca
      781 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
      841 caaaggatcc aacaacatgt tgatccccat tgctgtgggc ggtgccctgg cagggctggt
      901 cctcatcgtc ctcattgcct acctcattgg caggaagagg agtcacgccg gctatcagac
      961 catctcaggg tctggaccgg tcgccaccat ggtgagcaag ggcgaggaga ataacatggc
     1021 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     1081 gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct
     1141 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     1201 gtacggctcc aaggtctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc
     1261 cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca
     1321 cgttaaccag gactcctccc tgcaggacgg cgtgttcatc tacaaggtga agctgcgcgg
     1381 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1441 cgaggagcgg atgtaccccg aggacggcgc cctgaagagc gagatcaaga agaggctgaa
     1501 gctgaaggac ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc
     1561 cgtgcagctg cccggcgcct acatcgtcga catcaagttg gacatcgtgt cccacaacga
     1621 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1681 ggacgagctg tacaagtaag cggccgcgac tctagatcat aat
//
