LOCUS       sequence-85309-e        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-LC-Myosin-C-10
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgatgtcgag
      781 caagagagcc aaggccaaga ccaccaagaa gaggccccag agggctacgt ccaatgtctt
      841 cgccatgttt gaccagtccc agatccagga gtttaaggag gccttcaaca tgattgatca
      901 gaaccgcgat ggcttcattg ataaggagga cctgcacgac atgttggcct ctctggggaa
      961 gaaccccaca gacgagtacc tggagggcat gatgaacgag gcgccagggc ccatcaactt
     1021 caccatgttc ctcacaatgt ttggggagaa gctgaacggc acagaccccg aggatgtgat
     1081 ccgcaatgcc tttgcctgct ttgatgagga ggcctcaggc ttcatccacg aggaccacct
     1141 gagggagctg ctcaccacca tgggcgaccg attcacggat gaggaggtgg acgagatgta
     1201 ccgcgaggca cccattgata agaagggcaa cttcaactac gtggagttca ctcgcatcct
     1261 caaacacggc gccaaggaca aggacgacta aggatccacc ggatctagat aactgatcat
     1321 aatcagccat accacatt
//
