LOCUS       sequence-85310-e        1335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1335)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             586..1296
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     999..1018
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttat gtcgagcaag
       61 agagccaagg ccaagaccac caagaagagg ccccagaggg ctacgtccaa tgtcttcgcc
      121 atgtttgacc agtcccagat ccaggagttt aaggaggcct tcaacatgat tgatcagaac
      181 cgcgatggct tcattgataa ggaggacctg cacgacatgt tggcctctct ggggaagaac
      241 cccacagacg agtacctgga gggcatgatg aacgaggcgc cagggcccat caacttcacc
      301 atgttcctca caatgtttgg ggagaagctg aacggcacag accccgagga tgtgatccgc
      361 aatgcctttg cctgctttga tgaggaggcc tcaggcttca tccacgagga ccacctgagg
      421 gagctgctca ccaccatggg cgaccgattc acggatgagg aggtggacga gatgtaccgc
      481 gaggcaccca ttgataagaa gggcaacttc aactacgtgg agttcactcg catcctcaaa
      541 cacggcgcca aggacaagga cgacctggat ccaccggtcg ccaccatggt gagcaagggc
      601 gaggagaata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      661 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgaggcc
      721 tttcagaccg ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      781 ctgtcccctc agttcatgta cggctccaag gtctacatta agcacccagc cgacatcccc
      841 gactacttca agctgtcctt ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag
      901 gacggcggca ttattcacgt taaccaggac tcctccctgc aggacggcgt gttcatctac
      961 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1021 atgggctggg aggcctccga ggagcggatg taccccgagg acggcgccct gaagagcgag
     1081 atcaagaaga ggctgaagct gaaggacggc ggccactacg ccgccgaggt caagaccacc
     1141 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca tcgtcgacat caagttggac
     1201 atcgtgtccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1261 cactccaccg gcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
     1321 cagccatacc acatt
//
