LOCUS       sequence-85314-e        1867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1867)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-MAPTau-C-10
FEATURES             Location/Qualifiers
     source          1..1867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..734
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     440..459
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagaat aacatggcca
       61 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      121 tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga
      181 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      241 acggctccaa ggtctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct
      301 tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg
      361 ttaaccagga ctcctccctg caggacggcg tgttcatcta caaggtgaag ctgcgcggca
      421 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctccg
      481 aggagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag aggctgaagc
      541 tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg
      601 tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg
      661 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      721 acgagctgta caagtccgga agcggcgggt ccgggagcgg aagcgctgag ccccgccagg
      781 agttcgaagt gatggaagat cacgctggga cgtacgggtt gggggacagg aaagatcagg
      841 ggggctacac catgcaccaa gaccaagagg gtgacacgga cgctggcctg aaagctgaag
      901 aagcaggcat tggagacacc cccagcctgg aagacgaagc tgctggtcac gtgacccaag
      961 ctcgcatggt cagtaaaagc aaagacggga ctggaagcga tgacaaaaaa gccaaggggg
     1021 ctgatggtaa aacgaagatc gccacaccgc ggggagcagc ccctccaggc cagaagggcc
     1081 aggccaacgc caccaggatt ccagcaaaaa ccccgcccgc tccaaagaca ccacccagct
     1141 ctggtgaacc tccaaaatca ggggatcgca gcggctacag cagccccggc tccccaggca
     1201 ctcccggcag ccgctcccgc accccgtccc ttccaacccc acccacccgg gagcccaaga
     1261 aggtggcagt ggtccgtact ccacccaagt cgccgtcttc cgccaagagc cgcctgcaga
     1321 cagcccccgt gcccatgcca gacctgaaga atgtcaagtc caagatcggc tccactgaga
     1381 acctgaagca ccagccggga ggcgggaagg tgcaaatagt ctacaaacca gttgacctga
     1441 gcaaggtgac ctccaagtgt ggctcattag gcaacatcca tcataaacca ggaggtggcc
     1501 aggtggaagt aaaatctgag aagcttgact tcaaggacag agtccagtcg aagattgggt
     1561 ccctggacaa tatcacccac gtccctggcg gaggaaataa aaagattgaa acccacaagc
     1621 tgaccttccg cgagaacgcc aaagccaaga cagaccacgg ggcggagatc gtgtacaagt
     1681 cgccagtggt gtctggggac acgtctccac ggcatctcag caatgtctcc tccaccggca
     1741 gcatcgacat ggtagactcg ccccagctcg ccacgctagc tgacgaggtg tctgcctccc
     1801 tggccaagca gggtttgtga ggatccaccg gatctagata actgatcata atcagccata
     1861 ccacatt
//
