LOCUS       sequence-85318-e        6690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Burnette DT, Shao L, Ott C, Pasapera AM, Fischer RS, Baird MA, Der 
            Loughian C, Delanoe-Ayari H, Paszek MJ, Davidson MW, Betzig E, 
            Lippincott-Schwartz J
  TITLE     A contractile and counterbalancing adhesion system controls the 3D 
            shape of crawling cells.
  JOURNAL   J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. 
            Epub 2014 Apr 7.
  PUBMED    24711500
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201311104"; journalName: "J Cell Biol"; date: 
            "2014-04-14- 14"; volume: "205"; issue: "1"; pages: "83-96"
COMMENT     Sequence Label: mApple-MyosinIIA-N-18
FEATURES             Location/Qualifiers
     source          1..6690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5953..5962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5959..6669
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     6372..6391
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag gccaccatgg ctcagcaggc tgcagacaag
       61 tacctctatg tggataaaaa cttcatcaat aacccgctgg cccaagctga ctgggctgcc
      121 aagaagttgg tatgggtgcc ttccagcaag aatggctttg aaccagccag cctcaaggag
      181 gaggtgggag aagaggccat tgtagagctg gtagagaatg ggaagaaggt gaaggtgaac
      241 aaggacgaca tccagaagat gaacccaccc aagttctcca aggtggagga catggcagag
      301 ctcacgtgcc tcaacgaagc ttcggtgctg cacaacctca aggagcgata ctactcaggg
      361 cttatctaca cctattcagg cctgttctgt gtggtcatca acccttataa gaacctgccc
      421 atctactcag aggagatcgt ggagatgtac aagggcaaga agaggcacga gatgccaccc
      481 cacatctacg ccatcacaga tactgcctac cggagcatga tgcaggaccg ggaagatcag
      541 tccatcctgt gcacggggga gtctggagca gggaagacag agaacaccaa gaaagtcatc
      601 cagtacctgg cacatgtggc ctcctcacac aagagcaaga aggaccaggg ggagttggag
      661 cggcagctgc tacaggccaa ccctatccta gaggcctttg gaaacgccaa gacggtgaag
      721 aatgacaact cctctcgatt cggtaaattc attcgtatca actttgatgt caatggctac
      781 attgttggtg ccaacattga gacttatctt ctggagaaat ctcgtgctat ccgccaagcc
      841 aaagaggagc ggaccttcca catcttctac tacctgctgt ctggggccgg agaacacctg
      901 aagactgatc tcctgttgga gccatacaac aaataccgct tcctgtccaa cgggcacgtc
      961 accatccctg ggcagcagga caaggacatg ttccaggaga caatggaggc catgagaatt
     1021 atgggtatcc cagaggatga gcagatgggc ttgctgcggg tcatctctgg ggtccttcag
     1081 cttggcaaca ttgccttcaa gaaggagcgg aacactgacc aggcgtccat gccggacaac
     1141 acagctgctc aaaaggtgtc ccacctcctg gggatcaatg tgaccgactt caccagaggc
     1201 atcctcaccc cacgcatcaa ggtgggcaga gactatgtgc agaaggcgca gactaaagag
     1261 caggctgact ttgccattga ggccttggcc aaggctacct atgagcggat gttccgctgg
     1321 ctggtgcttc gcatcaacaa agctctggac aagaccaaga ggcagggcgc ctcatttatc
     1381 gggatactgg acatcgctgg ctttgaaatc tttgatctga actccttcga gcagctgtgc
     1441 atcaactaca ccaacgagaa gctgcagcag ctgttcaacc acaccatgtt catcctggag
     1501 caggaggagt accagcgaga gggcatcgag tggaacttca tcgacttcgg cctggacctg
     1561 cagccctgca tcgacctcat tgagaagccg gcgggtcccc caggcatcct ggccctgcta
     1621 gatgaggagt gctggtttcc taaggccact gacaagagct tcgtggagaa ggtggtgcag
     1681 gagcagggca cccaccccaa gttccagaag cccaagcaac tgaaggacaa ggctgatttc
     1741 tgcattatcc actatgccgg caaggtggac tataaagctg acgagtggct gatgaagaac
     1801 atggacccct tgaacgacaa catcgccacg ctgcttcacc agtcctcaga caagtttgtc
     1861 tctgagctgt ggaaggatgt ggatcggatc attggcttgg accaagtggc tggaatgtcc
     1921 gagacagcac tacctggtgc cttcaagacc cggaagggca tgttccgtac tgtcggacag
     1981 ctgtacaagg agcagctggc caagctcatg gccacgttga ggaataccaa ccccaacttc
     2041 gtgcgctgca tcattcccaa ccatgagaag aaggccggca aactggaccc gcacttggtg
     2101 ctggaccagc tgcgctgcaa tggcgtcctt gagggcatcc ggatctgccg ccagggcttt
     2161 cccaacaggg tggtcttcca ggagttccgg cagaggtatg agatcctcac ccccaactcc
     2221 atcccgaagg gcttcatgga tggcaagcaa gcgtgtgtgc tcatgatcaa agccttggag
     2281 cttgacagca acctgtaccg catcggccag agcaaagtgt tcttccgggc aggagtgctc
     2341 gcccacctgg aggaagagcg ggacctgaag atcaccgatg tcatcattgg cttccaggcc
     2401 tgctgcaggg gctacctggc caggaaggcc tttgccaaga ggcagcaaca gctgaccgcc
     2461 atgaaggtcc tacagaggaa ctgtgctgcg tacctcaggc tgcgcaactg gcagtggtgg
     2521 aggctcttca ccaaggtcaa gcccctgttg aactcaataa gacatgagga tgagctgtta
     2581 gccaaggagg cggaactgac aaaggttcga gagaaacatc tggctgcaga gaacaggctg
     2641 acagagatgg agacgatgca gtctcagctc atggcagaga agctgcagct tcaggagcag
     2701 ctgcaggcgg agacagagct gtgtgccgag gctgaggagc tccgggcccg tctgacagcg
     2761 aagaagcagg agctggagga gatatgccat gacctggagg ccagggtgga ggaggaggag
     2821 gagcgctgcc agtacctgca ggccgagaag aagaagatgc agcagaacat ccaggaactt
     2881 gaggagcagt tggaggagga ggagagcgcc cggcagaagc tgcagcttga gaaggtgacc
     2941 accgaggcca agctgaagaa actggaggag gaccagatca tcatggagga ccagaactgc
     3001 aaactggcca aggagaagaa actgctggaa gacagagtag ctgagttcac taccaacctc
     3061 atggaagagg aggagaagtc caagagcctg gccaagctca agaacaagca cgaggcaatg
     3121 atcaccgacc tggaagagcg cctccgtagg gaggagaagc agaggcagga gttggagaag
     3181 acccgtcgca agctggaggg agactccaca gacctcagtg accagattgc tgagctccag
     3241 gcgcagatag cagagctcaa gatgcagctg gccaagaagg aggaggagtt gcaggctgcc
     3301 ttggccagag tggaagaaga agctgctcag aagaatatgg ccctgaagaa gatccgagaa
     3361 ctggaaactc agatatctga gctccaggag gacctggagt cggagcgagc ctccaggaat
     3421 aaagccgaga agcagaaacg ggatctggga gaggagctgg aggcgctgaa gacagagctg
     3481 gaggacacgc tggactccac ggctgcccag caggagctga ggtcgaagcg tgagcaggag
     3541 gtgagcatcc tgaagaagac tctggaggac gaggccaaga cccatgaggc ccagatccag
     3601 gagatgaggc agaagcactc acaggctgtg gaggagctgg cagatcagtt ggagcagacg
     3661 aagcgggtaa aagctaccct tgagaaggcg aagcagaccc tggagaatga gcggggagag
     3721 ctggccaatg aggtgaaggc cctgctgcaa ggcaagggcg actcagagca caagcgcaag
     3781 aaggtggagg cgcagctgca agaactgcag gtcaagttca gcgagggaga gcgcgtgcga
     3841 accgaactgg ccgacaaggt caccaagctg caggttgaac tggacagtgt gaccggtctc
     3901 cttagccagt ctgacagcaa gtccagcaag cttacgaagg acttctctgc gctggagtcc
     3961 cagcttcagg acacacagga gttgctccag gaggagaacc ggcagaagct gagcctgagc
     4021 accaagctca agcagatgga ggatgagaaa aactccttca gggagcagct ggaggaggag
     4081 gaggaggcca agcgcaactt ggagaagcag atcgccacgc tccatgccca ggtgaccgac
     4141 atgaagaaga agatggagga cggtgtaggg tgcctggaga ctgcagagga ggcgaagcgg
     4201 aggcttcaga aggacttgga aggcctgagc cagcggcttg aggagaaggt ggctgcctac
     4261 gataagctgg agaagaccaa gacacggctg cagcaggagc tggacgacct gctggttgac
     4321 ctggaccacc agcggcagag cgtctccaac ctggaaaaga agcagaagaa gttcgaccag
     4381 ctcctagccg aggagaagac catctcggcc aagtatgcag aggagcgtga ccgagctgag
     4441 gctgaggccc gtgagaagga gacaaaggcg ctatcactgg cccgggcgct tgaggaggcc
     4501 atggagcaga aggcagagct ggagcggctc aacaagcagt tccgcacgga gatggaggac
     4561 ctcatgagct ccaaggatga cgtgggcaag agtgtccacg agctggagaa gtccaagcgg
     4621 gccttggagc agcaggtgga ggagatgaag acccagctgg aggagctgga ggatgagctg
     4681 caggccacgg aggatgccaa gctccgcctg gaggtgaacc tgcaggccat gaaggcccag
     4741 tttgagcggg atctgcaggg ccgggatgaa cagagcgagg agaagaagaa gcagctggtc
     4801 agacaggtgc gggagatgga ggcggagctg gaggatgaga ggaagcagcg ctccatggcc
     4861 atggccgcac gcaagaaact ggagatggat ctgaaggacc tggaggcaca cattgacaca
     4921 gccaataaga accgggaaga ggccatcaaa cagctgcgga agcttcaggc ccagatgaag
     4981 gactgcatgc gggagctgga cgacacgcgc gcctcccggg aggagatcct ggcgcaggcc
     5041 aaggagaatg agaagaagct gaagagcatg gaggccgaga tgattcagct gcaggaggaa
     5101 ctggcagctg ctgagcgtgc taagcgtcag gcccaacagg aacgggacga gctggctgat
     5161 gagatcgcca acagcagtgg caaaggggcc ctagcattag aggagaagcg gcgactggag
     5221 gcccgcattg ccctgctgga ggaggagctg gaggaggaac agggcaacac ggagctgatc
     5281 aacgatcggc tgaagaaggc caacctgcag atcgaccaaa taaacaccga cctgaacctg
     5341 gaacgcagcc acgcacagaa gaatgagaat gcgcgacagc agctggaacg ccagaacaag
     5401 gagctcaagg ccaagctgca ggaaatggag agtgctgtca agtccaaata caaggcctcc
     5461 atcgcggcct tggaggccaa aattgcacag ctggaggaac agctggacaa cgagaccaag
     5521 gagcgccagg cagcctccaa gcaggtgcgc cggacggaga agaagctgaa ggacgtgctg
     5581 ctgcaggtgg aggacgagcg gaggaacgcg gaacagttca aggaccaggc tgacaaggcg
     5641 tccacccgcc tgaagcagct taaacggcag ctagaggagg ctgaagagga ggcccagcgg
     5701 gccaatgcct cacgccggaa gctgcagcgt gagctggaag atgccacaga gaccgctgat
     5761 gctatgaacc gcgaggtcag ctccctgaag aacaaactga ggcgtgggga cctgccattt
     5821 gtcgtgactc gccgaattgt tcggaaaggc actggcgact gctcagacga ggaggtcgac
     5881 ggtaaagcag atggggccga tgccaaggca gctgaaggcg gaagcggcgg gagcgcaggg
     5941 gatccaccgg tcgccaccat ggtgagcaag ggcgaggaga ataacatggc catcatcaag
     6001 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     6061 gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc
     6121 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     6181 aaggtctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag
     6241 ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca cgttaaccag
     6301 gactcctccc tgcaggacgg cgtgttcatc tacaaggtga agctgcgcgg caccaacttc
     6361 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc cgaggagcgg
     6421 atgtaccccg aggacggcgc cctgaagagc gagatcaaga agaggctgaa gctgaaggac
     6481 ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     6541 cccggcgcct acatcgtcga catcaagttg gacatcgtgt cccacaacga ggactacacc
     6601 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     6661 tacaagtaag cggccgcgac tctagatcat
//
