LOCUS       sequence-85336-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             229..939
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     642..661
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcgccaccat gattcacacc
       61 aacctgaaga aaaagttcag ctgctgcgtc ctggtctttc ttctgtttgc agtcatctgt
      121 gtgtggaagg aaaagaagaa agggagttac tatgattcct ttaaattgca aaccaaggaa
      181 ttcctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
      241 ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      301 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      361 gcctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      421 atcctgtccc ctcagttcat gtacggctcc aaggtctaca ttaagcaccc agccgacatc
      481 cccgactact tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc
      541 gaggacggcg gcattattca cgttaaccag gactcctccc tgcaggacgg cgtgttcatc
      601 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      661 accatgggct gggaggcctc cgaggagcgg atgtaccccg aggacggcgc cctgaagagc
      721 gagatcaaga agaggctgaa gctgaaggac ggcggccact acgccgccga ggtcaagacc
      781 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
      841 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
      901 cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
      961 aatcagccat accacattg
//
