LOCUS       sequence-85338-e        1412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin Actin Binding Domain, Excitation: 568, Emission:
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1412)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Talin-ABM-10
FEATURES             Location/Qualifiers
     source          1..1412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      361 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
      541 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gatcctagaa
      781 gctgccaagt ccatcgctgc agccaccagt gcactggtaa aggctgcgtc agcagcccag
      841 agggagctgg tggctcaagg aaaggtgggc gccattccag ccaatgcact ggatgatggg
      901 cagtggtcgc agggcctcat ttccgctgcc cgtatggtgg ctgcggccac caacaatctg
      961 tgtgaagcag ctaacgcagc tgtccagggc catgctagcc aggagaaact catttcctca
     1021 gccaagcaag tagctgcctc cacagctcag ctcctggtag cttgcaaggt caaagcagat
     1081 caggactctg aggcaatgaa acggctccag gctgctggca atgcagtgaa gagggcctca
     1141 gataacctgg taaaggcggc ccagaaagct gcagccttcg aagaccagga gaatgagacg
     1201 gtggtggtga aggagaaaat ggttgggggc attgcccaga ttatcgcagc acaggaagag
     1261 atgcttcgga aggaacgaga gctggaagag gctcggaaaa agctcgccca gatccggcag
     1321 cagcagtaca agttcttgcc ttcagagctt cgagacgagc actaaggatc caccggatct
     1381 agataactga tcataatcag ccataccaca tt
//
