LOCUS       sequence-85352-e        2536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2536)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1781..1790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1787..2497
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2200..2219
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ccatggcggc
       61 cccccgcccg tctcccgcga tccccgtttc ggtctcggct ccggcttttt acgccccgca
      121 gaagaagttc ggccctgtgg tggccccaaa gcccaaagtg aatcccttcc ggcccaggga
      181 cagcgagcct cccccggcac ccggggccca gcgcgcacag atgggccggg tgggcgatat
      241 tcccccgccg cccccggaag actttcccct gcctccacct ccccttgctg gggatggcga
      301 cgatgcagag ggtgctctgg gaggtgcctt cccgccgccc cctcccccga tcgaggaatc
      361 atttccccct gcgcctctgg aggaggagat cttcccttcc ccgccgcctc ctccggagga
      421 ggagggaggg cctgaggccc ccataccgcc cccaccacag cccagggaga aggtgagcag
      481 tattgatttg gagatcgact ctctgtcctc actgctggat gacatgacca agaatgatcc
      541 tttcaaagcc cgggtgtcat ctggatatgt gcccccacca gtggccactc cattcagttc
      601 caagtccagt accaagcctg cagccggggg cacagcaccc ctgcctcctt ggaagtcccc
      661 ttccagctcc cagcctctgc cccaggttcc ggctccggct cagagccaga cacagttcca
      721 tgttcagccc cagccccagc ccaagcctca ggtccaactc catgtccagt cccagaccca
      781 gcctgtgtct ttggctaaca cccagccccg agggccccca gcctcatctc cggctccagc
      841 ccctaagttt tctccagtga ctcctaagtt tactcctgtg gcttccaagt tcagtcctgg
      901 agccccaggt ggatctgggt cacaaccaaa tcaaaaattg gggcaccccg aagctctttc
      961 tgctggcaca ggctcccctc aacctcccag cttcacctat gcccagcaga gggagaagcc
     1021 ccgagtgcag gagaagcagc accccgtgcc cccaccggct cagaaccaaa accaggtgcg
     1081 ctcccctggg gccccagggc ccctgactct gaaggaggtg gaggagctgg agcagctgac
     1141 ccagcagcta atgcaggaca tggagcatcc tcagaggcag aatgtggctg tcaacgaact
     1201 ctgcggccga tgccatcaac ccctggcccg ggcgcagcca gccgtccgcg ctctagggca
     1261 gctgttccac atcgcctgct tcacctgcca ccagtgtgcg cagcagctcc agggccagca
     1321 gttctacagt ctggaggggg cgccgtactg cgagggctgt tacactgaca ccctggagaa
     1381 gtgtaacacc tgcggggagc ccatcactga ccgcatgctg agggccacgg gcaaggccta
     1441 tcacccgcac tgcttcacct gtgtggtctg cgcccgcccc ctggagggca cctccttcat
     1501 cgtggaccag gccaaccggc cccactgtgt ccccgactac cacaagcagt acgccccgag
     1561 gtgctccgtc tgctctgagc ccatcatgcc tgagcctggc cgagatgaga ctgtgcgagt
     1621 ggtcgccctg gacaagaact tccacatgaa gtgttacaag tgtgaggact gcgggaagcc
     1681 cctgtcgatt gaggcagatg acaatggctg cttccccctg gacggtcacg tgctctgtcg
     1741 gaagtgccac actgctagag cccagacgga tccaccggtc gccaccatgg tgagcaaggg
     1801 cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     1861 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc
     1921 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     1981 cctgtcccct cagttcatgt acggctccaa ggtctacatt aagcacccag ccgacatccc
     2041 cgactacttc aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga
     2101 ggacggcggc attattcacg ttaaccagga ctcctccctg caggacggcg tgttcatcta
     2161 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     2221 catgggctgg gaggcctccg aggagcggat gtaccccgag gacggcgccc tgaagagcga
     2281 gatcaagaag aggctgaagc tgaaggacgg cggccactac gccgccgagg tcaagaccac
     2341 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga
     2401 catcgtgtcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     2461 ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa
     2521 tcagccatac cacata
//
