LOCUS       sequence-85356-e        2343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2343)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ALK5-N-13
FEATURES             Location/Qualifiers
     source          1..2343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1587..1596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1593..2303
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1738..1756)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2006..2025
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggac tcagatctgc caccatggag gcggcggtcg
       61 ctgctccgcg tccccggctg ctcctcctcg tgctggcggc ggcggcggcg gcggcggcgg
      121 cgctgctccc gggggcgacg gcgttacagt gtttctgcca cctctgtaca aaagacaatt
      181 ttacttgtgt gacagatggg ctctgctttg tctctgtcac agagaccaca gacaaagtta
      241 tacacaacag catgtgtata gctgaaattg acttaattcc tcgagatagg ccgtttgtat
      301 gtgcaccctc ttcaaaaact gggtctgtga ctacaacata ttgctgcaat caggaccatt
      361 gcaataaaat agaacttcca actactgtaa agtcatcacc tggccttggt cctgtggaac
      421 tggcagctgt cattgctgga ccagtgtgct tcgtctgcat ctcactcatg ttgatggtct
      481 atatctgcca caaccgcact gtcattcacc atcgagtgcc aaatgaagag gacccttcat
      541 tagatcgccc ttttatttca gagggtacta cgttgaaaga cttaatttat gatatgacaa
      601 cgtcaggttc tggctcaggt ttaccattgc ttgttcagag aacaattgcg agaactattg
      661 tgttacaaga aagcattggc aaaggtcgat ttggagaagt ttggagagga aagtggcggg
      721 gagaagaagt tgctgttaag atattctcct ctagagaaga acgttcgtgg ttccgtgagg
      781 cagagattta tcaaactgta atgttacgtc atgaaaacat cctgggattt atagcagcag
      841 acaataaaga caatggtact tggactcagc tctggttggt gtcagattat catgagcatg
      901 gatccctttt tgattactta aacagataca cagttactgt ggaaggaatg ataaaacttg
      961 ctctgtccac ggcgagcggt cttgcccatc ttcacatgga gattgttggt acccaaggaa
     1021 agccagccat tgctcataga gatttgaaat caaagaatat cttggtaaag aagaatggaa
     1081 cttgctgtat tgcagactta ggactggcag taagacatga ttcagccaca gataccattg
     1141 atattgctcc aaaccacaga gtgggaacaa aaaggtacat ggcccctgaa gttctcgatg
     1201 attccataaa tatgaaacat tttgaatcct tcaaacgtgc tgacatctat gcaatgggct
     1261 tagtattctg ggaaattgct cgacgatgtt ccattggtgg aattcatgaa gattaccaac
     1321 tgccttatta tgatcttgta ccttctgacc catcagttga agaaatgaga aaagttgttt
     1381 gtgaacagaa gttaaggcca aatatcccaa acagatggca gagctgtgaa gccttgagag
     1441 taatggctaa aattatgaga gaatgttggt atgccaatgg agcagctagg cttacagcat
     1501 tgcggattaa gaaaacatta tcgcaactca gtcaacagga aggcatcaaa atgggttccg
     1561 ggggtgcggg cggcggagga ccggtcgcca ccatggtgag caagggcgag gaggataaca
     1621 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1681 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1741 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1801 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     1861 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     1921 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     1981 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2041 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     2101 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     2161 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     2221 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     2281 gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac
     2341 atg
//
