LOCUS       sequence-85403-e        1323 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1323)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Centrin2-N-10
FEATURES             Location/Qualifiers
     source          1..1323
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      566..575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             572..1282
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(717..735)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     985..1004
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatggc ctccaacttt aagaaggcaa acatggcatc
       61 aagttctcag cgaaaaagaa tgagccctaa gcctgagctt actgaagagc aaaagcagga
      121 gatccgggaa gcttttgatc ttttcgatgc ggatggaact ggcaccatag atgttaaaga
      181 actgaaggtg gcaatgaggg ccctgggctt tgaacccaag aaagaagaaa ttaagaaaat
      241 gataagtgaa attgataagg aagggacagg aaaaatgaac tttggtgact ttttaactgt
      301 gatgacccag aaaatgtctg agaaagatac taaagaagaa atcctgaaag ctttcaagct
      361 ctttgatgat gatgaaactg ggaagatttc gttcaaaaat ctgaaacgcg tggccaagga
      421 gttgggtgag aacctgactg atgaggagct gcaggaaatg attgatgaag ctgatcgaga
      481 tggagatgga gaggtcagtg agcaagagtt cctgcgcatc atgaaaaaga ccagcctcta
      541 ttcgggaagc acggatccac cggtcgccac catggtgagc aagggcgagg aggataacat
      601 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      661 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      721 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      781 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      841 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      901 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      961 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1021 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1081 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1141 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1201 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     1261 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     1321 ttt
//
