LOCUS       sequence-85408-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     regulatory      266..275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             272..982
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(417..435)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     685..704
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
       61 cagatccgct agcgctaccg gactcagatc tggtaccctt gcagcttcca gttgtcagga
      121 agagaaggct cccatggatc ctgtggtggt gctggggctc tgtctctcct gtttgcttct
      181 cctttcactc tggaaacaga gctatggggg agggaagctt cgaattctgc agtcgacggt
      241 accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg aggataacat
      301 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      361 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      421 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      481 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      541 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      601 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      661 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
      721 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
      781 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
      841 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
      901 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
      961 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     1021 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tcccc
//
