LOCUS       sequence-85419-e        1659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1659)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-EB1-8
FEATURES             Location/Qualifiers
     source          1..1659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..79
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             909..1619
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1054..1072)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1322..1341
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctccaccgc ggtggcggcc
       61 gctctagaac tagtggatcc atggcagtga acgtatactc aacgtcagtg accagtgata
      121 acctaagtcg acatgacatg ctggcctgga tcaatgagtc tctgcagttg aatctgacaa
      181 agatcgaaca gttgtgctca ggggctgcgt attgtcagtt tatggacatg ctgttccctg
      241 gctccattgc cttgaagaaa gtgaaattcc aagctaagct agaacacgag tacatccaga
      301 acttcaaaat attacaagca ggttttaaga gaatgggtgt tgacaaaata attcctgtgg
      361 acaaattagt aaaaggaaag tttcaggaca attttgaatt cgttcagtgg ttcaagaagt
      421 tcttcgatgc aaactatgat ggaaaagact atgaccctgt ggctgctaga caaggtcaag
      481 aaactgcagt ggctccttcc cttgttgctc cagctctgaa taaaccgaag aaacctctca
      541 cttctagcag tgcagctccc cagaggccca tctcaacaca gagaaccgct gcggctccta
      601 aggctggccc tggtgtggtg cgaaagaacc ctggtgtggg caacggagac gacgaggcag
      661 ctgagttgat gcagcaggtc aacgtattga aacttactgt tgaagacttg gagaaagaga
      721 gggatttcta cttcggaaag ctacggaaca ttgaattgat ttgccaggag aacgaggggg
      781 aaaacgaccc tgtattgcag aggattgtag acattctgta tgccacagat gaaggctttg
      841 tgatacctga tgaagggggc ccacaggagg agcaagaaga gtatacccgg gatccaccgg
      901 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
      961 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1021 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     1081 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     1141 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1201 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     1261 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     1321 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     1381 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     1441 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     1501 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     1561 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa
     1621 gcggccgcga ctctagatca taatcagcca taccacatt
//
