LOCUS       sequence-85464-e        1688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1688)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MCPA2-N-18
FEATURES             Location/Qualifiers
     source          1..1688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      932..941
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             938..1648
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1083..1101)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1351..1370
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatggc ggatctggag gagcagttgt ccgatgagga
       61 gaaggttcgt atagcagcaa aattcatcat tcacgcccct cctggagaat ttaatgaggt
      121 ttttaatgat gttcgactat tgcttaataa tgataatctt ctcagggaag gagccgcaca
      181 tgcatttgca cagtataacc tggaccagtt tacaccagta aaaattgaag gctatgaaga
      241 tcaggttttg ataacagaac atggagactt gggaaatgga aaatttttgg atccaaaaaa
      301 cagaatctgt ttcaaatttg atcatttaag aaaggaagca actgatccaa ggccctacga
      361 agcagaaaat gccattgaat cctggagaac ttcagtagaa actgcactgc gagcttatgt
      421 aaaagagcat tatccgaatg gggtctgcac tgtgtatggc aaaaaagtag atgggcagca
      481 aaccattatt gcatgcatag aaagccatca gtttcaagca aaaaactttt ggaatggtcg
      541 ttggaggtca gagtggaagt ttacagtcac tccttccacc acacaggtgg ttggcatttt
      601 gaaaattcag gttcattatt atgaagatgg taatgttcag ctagtgagtc ataaagatat
      661 acaagattcc ctcacagtat ctaatgaggt gcaaacagca aaagaattta taaagattgt
      721 agaagctgca gaaaatgaat accagactgc catcagtgag aattatcaga cgatgtcgga
      781 cactaccttc aaagccttac gtcgacagtt gccagtgaca cgtactaaga tcgactggaa
      841 caagatcctt agctacaaga ttggcaaaga gatgcagaat gcaagtcgcg gaggcagtgg
      901 tggggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagga
      961 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1021 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1081 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1141 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1201 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1261 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     1321 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1381 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1441 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1501 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     1561 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1621 cggcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat
     1681 accacatt
//
