LOCUS       sequence-85470-e        2516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2516)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Moesin-C-14
FEATURES             Location/Qualifiers
     source          1..2516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..724
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(162..180)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     430..449
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
       61 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      121 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      181 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      241 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      301 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      361 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      421 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      481 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
      541 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      601 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      661 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      721 caagtccgga cccaaaacga tcagtgtgcg tgtgaccacc atggatgcag agctggagtt
      781 tgccatccag cccaacacca ccgggaagca gctatttgac caggtggtga aaactattgg
      841 cttgagggaa gtttggttct ttggtctgca gtaccaggac actaaaggtt tctccacctg
      901 gctgaaactc aataagaagg tgactgccca ggatgtgcgg aaggaaagcc ccctgctctt
      961 taagttccgt gccaagttct accctgagga tgtgtccgag gaattgattc aggacatcac
     1021 tcagcgcctg ttctttctgc aagtgaaaga gggcattctc aatgatgata tttactgccc
     1081 gcctgagacc gctgtgctgc tggcctcgta tgctgtccag tctaagtatg gcgacttcaa
     1141 taaggaagtg cataagtctg gctacctggc cggagacaag ttgctcccgc agagagtcct
     1201 ggaacagcac aaactcaaca aggaccagtg ggaggagcgg atccaggtgt ggcatgagga
     1261 acaccgtggc atgctcaggg aggatgctgt cctggaatat ctgaagattg ctcaagatct
     1321 ggagatgtat ggtgtgaact acttcagcat caagaacaag aaaggctcag agctgtggct
     1381 gggggtggat gccctgggtc tcaacatcta tgagcagaat gacagactaa ctcccaagat
     1441 aggcttcccc tggagtgaaa tcaggaacat ctctttcaat gataagaaat ttgtcatcaa
     1501 gcccattgac aaaaaagccc cggacttcgt cttctatgct ccccggctgc ggattaacaa
     1561 gcggatcttg gccttgtgca tggggaacca tgaactatac atgcgccgtc gcaagcctga
     1621 taccattgag gtgcagcaga tgaaggcaca ggcccgggag gagaagcacc agaagcagat
     1681 ggagcgtgct atgctggaaa atgagaagaa gaagcgtgaa atggcagaga aggagaaaga
     1741 gaagattgaa cgggagaagg aggagctgat ggagaggctg aagcagatcg aggaacagac
     1801 taagaaggct cagcaagaac tggaagaaca gacccgtagg gctctggaac ttgagcagga
     1861 acggaagcgt gcccagagcg aggctgaaaa gctggccaag gagcgtcaag aagctgaaga
     1921 ggccaaggag gccttgctgc aggcctcccg ggaccagaaa aagactcagg aacagctggc
     1981 cttggaaatg gcagagctga cagctcgaat ctcccagctg gagatggccc gacagaagaa
     2041 ggagagtgag gctgtggagt ggcagcagaa ggcccagatg gtacaggaag acttggagaa
     2101 gacccgtgct gagctgaaga ctgccatgag tacacctcat gtggcagagc ctgctgagaa
     2161 tgagcaggat gagcaggatg agaatggggc agaggctagt gctgacctac gggctgatgc
     2221 tatggccaag gaccgcagtg aggaggaacg taccactgag gcagagaaga atgagcgtgt
     2281 gcagaagcac ctgaaggccc tcacttcgga gctggccaat gccagagatg agtccaagaa
     2341 gactgccaat gacatgatcc atgctgagaa catgcgactg ggccgagaca aatacaagac
     2401 cctgcgccag atccggcagg gcaacaccaa gcagcgcatt gacgaatttg agtctatgta
     2461 aggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccata
//
