LOCUS       sequence-85501-e        3209 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3209)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Smoothened1-N-18
FEATURES             Location/Qualifiers
     source          1..3209
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2463..2472
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2469..3179
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2614..2632)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2882..2901
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cccatgctag tgacgtcaga tccgctagcg ccaccatggc cgctggccgc cccgtgcgtg
       61 ggcccgagct ggcgccccgg aggctgctgc agttgcttct gctggtactg ctggggggcc
      121 cgggccgggg ggcggccttg agcgggaacg tgaccgggcc tgggcctcac agcgccagcg
      181 ggagctcgag gagggacgtg ccggtgacca gccctccgcc gccgctgctg agccactgcg
      241 gccgggccgc ccactgcgag cctttgcgct acaacgtgtg cctgggctcg gcgctgccct
      301 acggagccac caccacgctg ctggctgggg actcggactc gcaggaggaa gcgcacggca
      361 agctcgtgct ctggtccggc ctccggaatg ccccccgctg ctgggcagtg atccagcccc
      421 tgctgtgtgc tgtctacatg cccaagtgtg agaatgaccg agtggagttg cccagccgta
      481 ccctctgcca ggccacccga ggcccctgtg ccattgtgga gcgggagcga gggtggcctg
      541 actttctgcg ttgcacaccg gaccacttcc ctgaaggctg cccaaacgag gtacaaaaca
      601 tcaagttcaa cagctcaggc caatgtgaag cacccttggt gcgaacagac aaccccaaga
      661 gctggtatga ggacgtggag ggctgtggga ttcagtgtca gaacccgctg ttcaccgagg
      721 ccgagcacca ggacatgcac agctacatcg cagccttcgg ggcggtcact ggtctctgca
      781 cgctcttcac actggccacc tttgtggctg actggcggaa ctccaatcgc taccctgcgg
      841 ttattctctt ctatgtcaat gcgtgtttct tcgtgggcag cattggctgg ctggcccagt
      901 tcatggatgg tgcccgccga gagattgttt gccgagcaga tggcaccatg agatttgggg
      961 agcccacctc cagtgagacc ctgtcctgtg tcatcatctt tgtcattgtg tactatgcct
     1021 tgatggctgg agtagtctgg ttcgtggtcc tcacctatgc ctggcacacc tccttcaaag
     1081 ccctgggcac cacctaccag cctctctcgg gcaagacatc ctatttccac ctgctcacgt
     1141 ggtcactccc ctttgtcctc acggtggcaa tcctggctgt ggctcaggta gatggagact
     1201 ccgtgagtgg catctgtttt gtaggctaca agaactatcg gtaccgtgct ggctttgtcc
     1261 tggccccaat tggcctggtg cttattgtgg gaggctactt cctcatcaga ggggtcatga
     1321 ctctgttctc catcaagagc aaccaccctg ggcttctgag tgagaaggca gccagcaaga
     1381 tcaacgagac catgctgcgc ctgggcattt ttggcttcct ggcctttggc tttgtgctca
     1441 tcaccttcag ctgccacttc tatgacttct tcaaccaggc tgagtgggag cgtagcttcc
     1501 gggactatgt gctatgccaa gccaacgtga ccatcgggct gcctaccaag aagcccattc
     1561 ctgactgtga gatcaagaat cggcccagcc tcctggtgga gaagatcaat ctatttgcca
     1621 tgtttggcac tggcattgcc atgagcacct gggtctggac caaggccacc ctgctcatct
     1681 ggaggcgcac ctggtgcagg ttgactgggc acagtgatga tgagcccaag agaatcaaga
     1741 agagcaagat gatcgccaag gccttctcta agcggcgtga gctgctgcag aacccgggcc
     1801 aggagctctc cttcagcatg cacactgtct cccatgatgg acctgttgcg ggtttggctt
     1861 ttgacctcaa tgaaccctca gctgacgtct cctctgcctg ggctcagcat gtcaccaaga
     1921 tggtggctcg gagaggagcc atattgcccc aggatgtgtc cgttacccct gtggcaactc
     1981 cagtgccacc agaagaacaa gccaacatgt ggctggttga ggcagagatc tccccagagt
     2041 tagagaagcg tttgggccgg aagaaaaagc ggaggaagag gaagaaggag gtgtgcccct
     2101 tgaggccagc ccctgagctt caccactctg cccctgttcc tgccaccagt gcagttcctc
     2161 ggctgcctca gctgcctcgg cagaagtgcc tggtagctgc aaacgcctgg ggaacagggg
     2221 agtcctgccg acagggagcc tggactctag tctccaaccc cttctgccca gagcctagtc
     2281 cccatcaaga tccatttctc cctggtgcct cagccccccg ggtctgggct cagggccgcc
     2341 tccaggggct gggatccatt cattcccgca ctaacctaat ggaggctgag atcttggatg
     2401 cagactcgga cttctctcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     2461 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     2521 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     2581 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     2641 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     2701 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     2761 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     2821 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2881 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2941 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     3001 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     3061 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     3121 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag
     3181 cggccgcgac tctagatcat aatcagcca
//
