LOCUS       sequence-85503-e        6376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Actin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW, 
            Lambright DG, Luna EJ
  TITLE     The membrane-associated protein, supervillin, accelerates 
            F-actin-dependent rapid integrin recycling and cell motility.
  JOURNAL   Traffic. 2010 Jun;11(6):782-99. doi: 
            10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17.
  PUBMED    20331534
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01062.x"; journalName: "Traffic"; date: 
            "2010-06"; volume: "11"; issue: "6"; pages: "782-99"
COMMENT     Sequence Label: mCherry-Supervillin-45
FEATURES             Location/Qualifiers
     source          1..6376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattctgca
      781 gtcgacggta ccttcgatgc tcagctttta gctgcccttc ctaaagttgc agaattaaga
      841 aaaatctttg aaccaaagag gaaagaattt ttagaaatga aaagaaaaga aagaattgcc
      901 cggcgcttag aaggaattga aaccgacacg cagcccatcc tcttgcagag ctgcacgggc
      961 ttggtgactc atcgcctgct ggaggaggac acacccagat acatgcgtgc cacagacccg
     1021 gccagcccgc acattggtcg atcaaatgaa gaagaagaaa cttcagattc ttcactagaa
     1081 aagcagactc gatccaaaca gtgcacagaa acctcaggca tccatgctga ctcgccctac
     1141 agttcaggca tcatggacac ccagagcctc gagtccaaag ccgaaagaat cgccaggtac
     1201 aaagcagaga gaagacggca gctggcagaa aagtatgggc tcaccctgga tccggaagca
     1261 gactctgaaa ctccatctcg atacagcagg tccagaaagg accccgaggc tgcggagaaa
     1321 aggggagtaa gaagcgagcg atcggccgag tccagcagag acgccggctc atcctactcc
     1381 aggactgagc tctcggggct caggacctgc gtggctgagt ccaaggacta yggcctgcac
     1441 cggagtgacg gtgtttccga cacagaggtg ctgctcaatg cagaaaacca aagacgcggt
     1501 caagagccca gtgccaccgg gctggcccga gacctgcccc ttgcagggga ggtctcctct
     1561 tctttctcat tctccgggcg agactctgcc ctcggtgaag tgccaaggtc cccaaaggca
     1621 gtgcacagcc tgcccagtcc atcgcctggg cagccggcct ccccaagcca ctccaccagt
     1681 gacctgccac tccctgcgga ggctcgagcc aggtcaactt caaattcaga aatgccagct
     1741 gccgaggatg aagaaaaggt cgatgagcga gcgaggctga gcgtcgctgc taagaggctg
     1801 ctcttcaggg agatggagaa gtcgtttgac gagaagagcg tcccaaagcg ccgctcccgc
     1861 aatgcggccg tggagcagag gctgcggcgc ctccaggacc ggtcccacac gcagcccgtc
     1921 accaccgagg aggtggtcat tgcagccaca ttgcaggcgt ctgctcacca aaaggcacta
     1981 gccagagacc agacaaacga gagcaaagat tctgctgagc agggagaacc tgactcctcc
     2041 actctgagct tagcggagaa gctggccttg tttaacaaac tatctcagcc ggtctcaaaa
     2101 gccatttcaa cacggaacag attagacatg agacagagga ggatgaatgc tcgttatcaa
     2161 acccagccgg tcacgcttgg agaagtggaa caggtgcaga gtggcaagct catggctttc
     2221 tctcccacca ttaacacttc cgtgtccacc gtggcatcta ccgtcccccc catgtatgca
     2281 ggaaatcttc ggacaaagcc acttccagat gacagctttg gtgccactga gcagaagttt
     2341 gcttcttcgc tagaaaactc cgactcccca gttagaagca tcctgaaatc ccaaggctgg
     2401 cagccctcgg tcgagggtgc tgggagcaaa gcaatgttga gagaatttga agagacagaa
     2461 cgcaagggag gcttgacagg tggagatggc ggggttacaa agtatgggtc cttcgaggaa
     2521 gcagagctgt cctaccccgt cctcagcaga gtccgggagg gagacaacca taaagaggcc
     2581 atctatgccc ttccgaggaa aggaagcctg gagctcgccc atcctcccat cgcccagctt
     2641 ggtgatgacc tgaaggaatt ttccacccca aagagcacca tgcaggcaag cccagactgg
     2701 aaggagaggc agctctttga agagaaggtg gacttggaga atgtcacaaa gaggaagttt
     2761 tcgctgaaag ccgcggagtt cggggaaccc acttctgagc agaccggcgc ggctgctggg
     2821 aaaccggctg ctccgactgc aaccccggtg tcctggaagc cgcaggatcc ctccgaacag
     2881 cctcaggaga agcggtatca gagcccgtgt gcgatgtttg ctgctggaga gatcaaagcc
     2941 ccggcggtgg agggcagcct cgactccccc agcaaaacca tgtccattaa agaaagatta
     3001 gcactgttga agaagagcgg tgaggaagac tggagaaaca gactcaacag aaagcaggaa
     3061 tacggcaaag cctccatcac cagcagcctg cacatccaag agacggagca gtcactcaag
     3121 aagaagcggg tcacagaaag tcgagagagc cagatgacta ttgaagagag aaagcatctc
     3181 atcaccgtga gagaggacgc ctggaagacc aggggcaaag gagcagccaa cgactctacc
     3241 cagtttactg tggccggcag gatggtgaag cgaggtctgg cgtcccccac tgccatcacc
     3301 ccggtagcat cccctgtttc cagcaaagca aggggcacta caccagtttc cagacccctg
     3361 gaagatatcg aagccagacc agacatgcag ttagagtcgg acctcaagct ggacaggctg
     3421 gaaaccttcc taagaaggct gaataacaaa gttggtggga tgcaagaaac agtcctcact
     3481 gtcactggga aatcggtgaa agaggtgatg aagccggacg atgatgaaac ctttgccaag
     3541 ttttaccgca gcgtggactc ctctctaccg agaagccccg tagagctgga cgaggatttc
     3601 gatgtcattt ttgatcctta tgcccccagg ttgacatcct cggtggctga gcacaagcgt
     3661 gccgtgcggc ccaagcgccg ggtccaggct tccaaaaacc ccctgaagat gctggccgcg
     3721 agggaagatc tcctgcagga gtacactgag cagagactga acgtggcctt cgtggagtca
     3781 aagcggatga aagtcgaaaa gttgtccgcc aactccagct tctcagaagt caccctggcg
     3841 gggttagcca gcaaagaaaa cttcagcaac gtcagcctgc ggagcgtcaa cctgacggaa
     3901 cagaattcca acaacagtgc agtgccctac aagaagctga tgctgctgca ggttaaagga
     3961 agaagacacg tgcagacgcg gctggtggag cctcgcgccc cctccctcaa cagcggggac
     4021 tgcttcctcc tgctctcgcc ccatcactgc ttcctgtggg tcggagagtt cgccaacgtg
     4081 atcgagaagg cgaaggcctc agaacttgcg agtttaattc agaccaagag ggaacttggt
     4141 tgtagagcaa cttacatcca gactgttgaa gaaggaatta atacacacac acatgcagcc
     4201 aaagacttct ggaagcttct gggcggccaa gccagttacc aatccgccgg agacccaaag
     4261 gaggatgagc tctatgaaac ggccataata gagaccaact gcatttaccg tctgatggat
     4321 gacaaactcg ttcctgatga tgactactgg gggaagatcc caaagtgctc cctcctgcaa
     4381 tcaaaagagg tactggtgtt tgattttggg agtgaagttt acgtgtggca tggaaaagaa
     4441 gtcacattag cacaacggaa aatcgccttt cagctggcaa agcacttgtg gaatggaacc
     4501 tttgactacg agaattgtga cattaacccg ctggatcctg gggaatgcaa tcccctcatt
     4561 cccaggaaag gacaggggcg gcccgactgg gcaatatttg ggagacttac agaacacaat
     4621 gagacgatct tgttcaaaga gaaattcctt gattggacgg aactgaagag acctaacgag
     4681 aagaacgcca gcgaactcgc gcagcacaag gacgatgccc gggcagaggt caagccttac
     4741 gacgtgacgc ggatggtgcc cgtgccccag acaacagccg gcaccgtgct ggatggggtc
     4801 aacgtgggcc gaggctacgg gctggtggaa ggggacgacc ggaggcagtt cgagatcgcc
     4861 agcatctcag tggacgtctg gcacatcctg gagttcgact acagcaggct ccccaagcag
     4921 agcatcgggc agttccacga aggcgatgcc tacgtggtga agtggaagtt catcgtgagc
     4981 actgcagtgg gaagccggca gaagggggag cactcggtca gggtggctgg caaagagaaa
     5041 tgtgtctact tcttctggca aggccgccag tcgaccgtga gtgagaaggg cacatcagct
     5101 ctgatgacag tggagctgga cgaggaaagg ggggcccagg tccaggtcct gcagggcaag
     5161 gagcccccct gtttcctgca gtgcttccag ggggggatgg tggtccactc cgggagacgg
     5221 gaggaggagg aagagaacac acagagcgag tggcggctgt actgtgtgcg cggagaggtg
     5281 cctgtggagg ggaacttgct ggaggtggcc tgtcactgca gcagcctcag gtctaggacg
     5341 tccatggttg tcctcaacgt ccacaaagcc ctcatctacc tgtggcatgg atgcaaagcc
     5401 caggcccaca cgaaggaggt cggacggact gcagccaata aaatcaagga ccaatgtccc
     5461 ctggaggccg ggctgcacag tagcagcaaa gtgacaattc atgagtgtga tgaaggctcg
     5521 gagcccctgg ggttctggga cgctctgggg aggagagacc ggaaagccta cgactgcatg
     5581 cttcaagatc ctggaaattt taacttcaca ccccgcctgt tcatcctcag cagctcctct
     5641 ggtgacttct cagccacaga gttcatgtat cccgcccgag acccctctgt ggtcaactct
     5701 atgcccttcc tgcaggaaga cctatacagt gccccacagc cagcactctt ccttgttgac
     5761 aatcaccacg aggtgtacct ctggcaaggc tggtggccca tcgagaacaa gatcacgggc
     5821 tccgcgcgca tccgctgggc ctcggaccgg aagagcgcca tggagacagt gctgcagtac
     5881 tgccgaggga aaaacctgaa gaagccgccc cccaagtctt acctcatcca cgctggcctg
     5941 gaacccctga ccttcaccaa catgttcccc agctgggagc acagagaaga cattgcagag
     6001 atcacggaga tggacacgga agtttcaaat cagatcaccc tagtggagga tgtcttagcc
     6061 aaactgtgta agaccattta cccactggcc gacctcctag ccaggccgct ccctgaggga
     6121 gttgaccctc tgaagctgga gatctatctc accgacgagg acttcgagtt tgcactagac
     6181 atgacccgag acgagtacaa tgcactgccc gcttggaagc aggtgaacct gaagaaagcg
     6241 aaaggtctgt tctgatcagg agatgctgga ggaacctggg tggtcacttt caatactgat
     6301 ggaccaggaa aatggatgtt taatcgaatt cccgcggcct tctagataac tgatcataat
     6361 cagccatacc acattt
//
