LOCUS       sequence-85506-e        7165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7165)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Talin-R-15
FEATURES             Location/Qualifiers
     source          1..7165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(180..198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      181 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      541 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagcta gcttcgaatt
      781 cgccaccatg cagcagtaca accgagtggg gaaagtggag cacggctctg tggctctgcc
      841 agccatcatg cgctctggag cctctggtcc tgagaatttc caggtgggga gcatgccacc
      901 tgcccagcag cagatcacca gtggccagat gcaccgagga cacatgccac ctctgacttc
      961 agcccagcag gcgctcactg gaaccattaa ctccagcatg caggctgtgc aggctgccca
     1021 ggccactctg gatgactttg agactctacc ccctcttggc caggatgctg cctccaaagc
     1081 ttggcgtaag aacaagatgg atgaatcaaa gcatgagatc cactcccagg tagatgccat
     1141 cacagctggc actgcctctg tggtaaacct gacagcagga gatcctgcag agacagacta
     1201 tacagcagtg ggctgtgcag tcaccaccat ctcttccaac ctgacggaga tgtcccgtgg
     1261 ggtgaagcta ttggctgccc tgctggagga tgaaggcggc aatggccggc cccttctgca
     1321 agcagcaaag ggccttgcag gggctgtgtc ggaactgctt cgcagtgctc agcccgccag
     1381 cgctgagcct cgtcagaacc tgctgcaggc agccgggaac gtgggccagg ccagtgggga
     1441 gctgttgcag caaattgggg aaagtgacac tgacccccac ttccaggatg ttctaatgca
     1501 gctagcaaac gcagtggcaa gtgctgcagc cgccctggtc ctcaaggcca agagtgtggc
     1561 ccagcgcaca gaggattccg ggcttcagac ccaagtgatt gctgcagcta cgcaatgtgc
     1621 tctgtccact tcccagctgg tggcctgtac caaggtggta gcacctacaa tcagctcacc
     1681 tgtgtgccaa gaacagctag tcgaggctgg gcgactggtg gccaaagctg tggagggctg
     1741 tgtgtctgcc tcgcaagcag ctacagaaga tggacagctg cttcgagggg taggagcagc
     1801 agccacggct gtcacccagg ccctcaatga gctgctgcag cacgtgaagg cccacgccac
     1861 cggagctggg cctgccggtc ggtatgacca agccactgat accatcctca ccgtcactga
     1921 aaacatcttc agctccatgg gtgatgctgg ggagatggtg cgccaggccc gcatcctggc
     1981 ccaagccacc tcagacctgg tcaatgctat caaggccgat gctgaggggg agagtgatct
     2041 ggagaactct agaaagctcc tgagtgctgc caagatcctc gctgatgcca ccgccaagat
     2101 ggtggaggcg gccaagggag cagccgccca ccctgacagt gaggaacagc agcagcgact
     2161 gcgtgaagca gctgaggggc ttcgcatggc caccaatgca gctgcgcaga acgccatcaa
     2221 gaagaagttg gtgcagcgcc tggagcatgc agccaagcaa gctgcagcct ctgcaacaca
     2281 gaccattgct gcagcccaac atgcagcctc tgcccccaag gcctctgcgg gcccccagcc
     2341 cctactggtg cagagctgta aggccgtggc agagcagatt ccgttgctgg tgcagggtgt
     2401 ccgaggaagt caagctcagc ctgacagccc tagtgctcag ctcgccctca ttgctgccag
     2461 ccagagcttc ctgcagccag gtgggaagat ggtggcagcg gcaaaagcct cagtgccaac
     2521 aattcaggac caggcttcag cgatgcagct gagtcagtgc gcaaagaacc taggcactgc
     2581 cctggccgag ctgcgcactg ctgctcagaa ggctcaggag gcatgtggac ctttggagat
     2641 ggattctgca ctgagtgttg tacaaaatct agagaaagac ctacaggaaa taaaagcagc
     2701 agctcgagat ggcaagctta agcccttacc tggggaaaca atggagaagt gtacccaaga
     2761 tcttggcaac agcaccaaag cggtgagctc tgccatcgcc aagctgctgg gggagattgc
     2821 ccagggcaat gagaactatg caggtattgc agctcgggat gtagctggtg gactaaggtc
     2881 actagcccag gctgcgcggg gtgtggctgc gctgacatca gatcctgcag tgcaggccat
     2941 tgtgcttgac acagccagcg acgttctgga caaggccagc agcctcattg aagaggcaaa
     3001 aaaagcatct ggacacccag gggacccaga aagccagcaa aggcttgctc aggtagctaa
     3061 agcagtgacc caagccctga accgctgtgt cagctgcctg cctggccaaa gagacgtgga
     3121 taatgcccta cgagccgttg gagatgccag caagcgactc ctgagtgact tgcttcctcc
     3181 aagcacgggg acatttcaag aagcacagag ccgattgaat gaagctgcag ctgggttaaa
     3241 tcaggccgcc acagaactgg tgcaagcctc ccgaggaact cctcaggacc tggctcgggc
     3301 ctccggccga tttggacagg acttcagcac cttcctggaa gctggtgtgg agatggccgg
     3361 acaggccccg agccaggagg accgagccca ggtggtgtcc aacctgaagg gcatatccat
     3421 gtcttcaagc aaacttctcc ttgccgctaa ggccttgtcc acagaccctg cttctcccaa
     3481 cctcaagagt cagctggctg cggctgcccg ggcagtgacg gacagcatca accagctcat
     3541 caccatgtgc acccagcagg cacctggcca gaaggagtgt gacaatgcac ttcggcagct
     3601 agagacagtc cgagaactcc tggagaatcc agtccagccc atcaacgaca tgtcctactt
     3661 cggttgcttg gacagtgtca tggagaactc caaggtccta ggtgaggcca tgactggcat
     3721 ctcccaaaat gccaagaatg gaaatctgcc ggagtttgga gatgccattg ccacagcctc
     3781 caaggctctc tgtggcttca cggaggcagc cgcacaggca gcatatctag ttggtgtctc
     3841 tgaccccaac agccaagctg gacagcaagg actggtggaa cccacacagt ttgcccgtgc
     3901 aaaccaggca attcagatgg cctgtcagag tctgggggag cctggctgta cccaggccca
     3961 ggtgttatct gcagccacta ttgtagccaa acacacatct gcattgtgta acagctgtcg
     4021 cctggcttcc gctaggactg ccaatcccac tgccaagcgc cagtttgtac agtcagccaa
     4081 ggaggtggcc aacagtacag ccaatcttgt caagaccatc aaggcactag atggggactt
     4141 cacagaagag aaccgtgccc agtgccgagc agccacagcc cctctgctgg aagctgtgga
     4201 taacctgagt gcctttgcct ccaaccctga gttctccagc gtccctgccc agatcagccc
     4261 tgagggcaga gcagccatgg agcccattgt aatctctgct aagacaatgt tggagagtgc
     4321 tggagggctc atccagacag ctcgggcgtt agcagtcaat ccccgagacc ccccacgttg
     4381 gtctgtgcta gctggccact ctcggactgt ctcagattcc atcaagaaac ttattacaag
     4441 catgagggac aaagccccag ggcagctgga gtgtgagaca gccattgcgg ctctgaacag
     4501 ctgcttgcgg gacctagatc aggcttcgct tgccgctgtc agccagcagc ttgctccccg
     4561 tgaaggaatc tctcaagagg ctttgcacac ccagatgctc actgcagtgc aagaaatttc
     4621 tcatctcatt gagccgctgg ccagcgctgc tcgggctgaa gcctcccagc tgggacacaa
     4681 ggtgtcccaa atggcccagt actttgaacc actcaccctg gctgcagtgg gtgctgcgtc
     4741 taagaccctg agccacccgc agcagatggc acttctggac cagactaaaa cgttggcaga
     4801 gtctgccttg cagttgctat atactgccaa ggaggctggt ggtaacccca agcaagcagc
     4861 acacacccag gaagccctgg aggaggctgt gcagatgatg acagaggccg tagaagacct
     4921 gacgacgacc ctcaacgaag cagccagtgc tgcaggagtc gttggcggca tggtggactc
     4981 tatcactcag gccatcaacc agctagatga aggacctatg ggtgacccag aaggctcatt
     5041 cgtagattac cagacaacca tggtgaggac agccaaggcc attgctgtca ctgttcagga
     5101 gatggtaacc aagtcaaaca ccagccctga agagctaggc cctcttgcca accagctgac
     5161 cagtgactat ggccgactgg cctcacaagc caagcctgca gctgtggctg ctgaaaatga
     5221 agagataggc gctcacatca aacaccgagt acaggagctg ggccatggct gctctgctct
     5281 ggtcaccaag gcaggtgcct tgcagtgtag ccccagtgat gtctacacca agaaggagct
     5341 catagagtgt gcccgcagag tgtcagagaa ggtctcccat gttctggctg cactccaggc
     5401 tgggaatcgt ggtacccagg cctgcattac agcagccagt gctgtgtctg gtatcattgc
     5461 tgacctcgac accaccatca tgttcgctac tgctggcaca cttaaccgtg agggtgctga
     5521 aacttttgct gaccaccggg agggtatctt aaagacagca aaggttcttg tggaggacac
     5581 caaggtccta gtgcagaatg cagctgggag ccaggagaag ttggcacaag ccgcccagtc
     5641 ctccgtggcc accattaccc gcctcgctga tgtggtcaag ctcggtgcag ccagcctagg
     5701 agccgaagac cctgaaactc aggtggtgct gatcaatgca gtaaaggacg tagccaaggc
     5761 cctgggtgac ctcatcagcg ctacgaaggc tgcagcgggc aaagttgggg atgaccctgc
     5821 agtgtggcag ctcaagaact ctgccaaggt gatggtgacc aatgtgacat cattgctcaa
     5881 gacagtgaag gctgtggaag atgaggccac caaaggcaca cgggccctag agaagacctc
     5941 tacccctgaa gacttcatcc gaatgaccaa gggtattact atggcaacag ccaaagccgt
     6001 tgctgctggc aattcctgtc gacaggaaga tgtcattgcc acagccaatc tgagccgacg
     6061 ggctattgcg gacatgcttc gggcttgcaa ggaagcagct ttccacccag aagtggcgcc
     6121 tgatgtacgg ctccgagccc tgcactatgg ccgggagtgt gccaatggtt acctggaact
     6181 gctggaccac gtgctgctga cccttcagaa gccaaaccca gacctgaagc agcagctaac
     6241 tggacactca aagcgggttg ctggctctgt gactgagctc atccaggctg ctgaagccat
     6301 gaaaggaaca gagtgggtgg acccagagga ccctactgtc attgctgaga atgagctctt
     6361 gggagccgca gccgccatcg aggctgcagc caagaagctg gagcagctga agcccagggc
     6421 caaacccaag gaggcggatg agtccttgaa ctttgaggaa caaatcctag aagctgccaa
     6481 gtccatcgct gcagccacca gtgcactggt aaaggctgcg tcagcagccc agagggagct
     6541 ggtggctcaa ggaaaggtgg gcgccattcc agccaatgca ctggatgatg ggcagtggtc
     6601 gcagggcctc atttccgctg cccgtatggt ggctgcggcc accaacaatc tgtgtgaagc
     6661 agctaacgca gctgtccagg gccatgctag ccaggagaaa ctcatttcct cagccaagca
     6721 agtagctgcc tccacagctc agctcctggt agcttgcaag gtcaaagcag atcaggactc
     6781 tgaggcaatg aaacggctcc aggctgctgg caatgcagtg aagagggcct cagataacct
     6841 ggtaaaggcg gcccagaaag ctgcagcctt cgaagaccag gagaatgaga cggtggtggt
     6901 gaaggagaaa atggttgggg gcattgccca gattatcgca gcacaggaag agatgcttcg
     6961 gaaggaacga gagctggaag aggctcggaa aaagctcgcc cagatccggc agcagcagta
     7021 caagttcttg ccttcagagc ttcgagacga gcactaaaaa agccctgtgt atttaatgca
     7081 gacccagccc agagactgtg cctgccacta ccaaagcctt ctgggctgct caggacccaa
     7141 gctgcccaac cccagcactc cccca
//
