LOCUS       sequence-85509-e        6011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6011)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Tensin1-C-14
FEATURES             Location/Qualifiers
     source          1..6011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..724
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(162..180)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     430..449
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
       61 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      121 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      181 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      241 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      301 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      361 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      421 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      481 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
      541 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      601 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      661 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      721 caagtccgga agtgctggga gcgcaagcgg aagcggaggc tccggtagtg tgagccggac
      781 catggaggac agctgtgagc tggacctggt gtacgtcaca gagaggatca tcgctgtctc
      841 cttccccagc acagccaatg aggagaactt tcggagcaac ctccgtgagg tggcgcagat
      901 gctcaagtcc aaacatggag gcaactacct gctgttcaac ctctctgagc ggagacctga
      961 catcacgaag ctccatgcca aggtactgga atttggctgg cccgacctcc acaccccagc
     1021 cctggagaag atatgcagca tctgtaaggc catggacaca tggctcaatg cagaccctca
     1081 caatgtcgtt gttctacaca acaagggaaa ccgaggcagg ataggagttg tcatcgcggc
     1141 ttacatgcac tacagcaaca tttctgccag tgcggaccag gctctggatc ggtttgcaat
     1201 gaagcggttc tatgaggata agattgtgcc cattggccag ccatcccaaa gaaggtacgt
     1261 gcattacttc agtggcctgc tctccggctc catcaaaatg aacaacaagc ccttgtttct
     1321 gcaccacgtg atcatgcacg gcatccccaa ctttgagtct aaaggaggat gtcggccatt
     1381 tctccgcatc taccaggcca tgcaacctgt gtacacatct ggcatctaca acatcccagg
     1441 agacagccag actagcgtct gcatcaccat cgagccagga ctgctcttga agggagacat
     1501 cttgctgaag tgctaccaca agaagttccg aagcccagcc cgagacgtca tcttccgtgt
     1561 gcagttccac acctgtgcca tccatgacct gggggttgtc tttgggaagg aggaccttga
     1621 tgatgctttc aaagatgatc gatttccaga gtatggcaaa gtggagtttg tattttctta
     1681 tgggccagag aaaattcaag gcatggagca cctggagaac gggccgagcg tgtctgtgga
     1741 ctataacacc tccgaccccc tcatccgctg ggactcctac gacaacttca gtgggcatcg
     1801 agatgacggc atggaggagg tggtgggaca cacgcagggg ccactagatg ggagcctgta
     1861 tgctaaggtg aagaagaaag actccctgca cggcagcacc ggggctgtta atgccacacg
     1921 tcctacactg tcggccaccc ccaaccacgt ggaacacacg ctttctgtga gcagcgactc
     1981 gggcaactcc acagcctcca ccaagaccga caagaccgac gagcctgtcc ccggggcctc
     2041 cagtgccact gctgccttga gtccccagga gaagcgggag ctggaccgcc tgctgagtgg
     2101 ctttggctta gagcgagaga agcaaggcgc catgtaccac acccagcacc tcaggtcccg
     2161 cccagcaggg ggctcggctg tgccctcctc tggacgccac gttgtcccag cccaggttca
     2221 tgtcaatggt ggggcgttag catctgagcg ggagacagac atcctggacg atgaattgcc
     2281 aaaccaggat ggtcacagtg cgggcagcat gggcacactc tcttctctgg acggggtcac
     2341 caacaccagt gaggggggct acccagaggc cctgtcccca ctgaccaacg gtctggacaa
     2401 gtcctacccc atggagccta tggtcaatgg aggaggctac ccctacgagt ctgccagccg
     2461 ggcggggcct gcccatgctg gccacacggc ccccatgcgg ccctcctact ctgcacagga
     2521 gggtttagct ggctaccaga gggaggggcc ccacccagcc tggccacagc cagtgaccac
     2581 ctcccactat gcccatgacc ccagcggtat gttccgctct caatcctttt cggaagctga
     2641 accccagctg cccccagctc cggtccgagg gggaagcagc cgggaggctg tgcaaagggg
     2701 actgaactcg tggcagcagc agcagcagca gcagcagcag cctcgcccac ctccacgcca
     2761 gcaggaaaga gcccacttgg agagtcttgt agccagcagg cccagccctc agccattggc
     2821 agagaccccc atccccagtc tccctgagtt cccgcgagca gcctcccagc aggagattga
     2881 acagtccatc gaaacactca atatgctgat gctggacctg gagccagcct ccgctgctgc
     2941 cccactacac aagtcccaga gtgtccccgg ggcctggcca ggggcttctc cactctcctc
     3001 ccagcccctc tctgggtcct cccgtcagtc ccatccactg acccagtcca gatatggcta
     3061 tatccccagt gggcattcgt tgggaacccc tgagccagcc ccacgggcct ctctggagtc
     3121 tgtccctcct ggcaggtctt actcacctta tgactatcag ccatgtttgg ctgggcctaa
     3181 ccaggatttc cattcaaaga gcccagcctc ttcctccttg cctgccttcc ttccgaccac
     3241 ccacagccct ccagggcctc agcaaccccc agcctctctc cctggcctca ctgctcagcc
     3301 tctgctctca ccaaaggaag cgacttcaga cccctcccgg actccagagg aggagccatt
     3361 gaatttagaa gggctggtgg cccacagggt agcaggggta caggctcggg agaagcagcc
     3421 tgcagagccc ccagcccctc tgcggaggcg ggcggccagt gatggacagt atgagaacca
     3481 gtctccagaa gccacatccc ctcgtagccc tggggttcgc tcccctgtcc agtgtgtctc
     3541 cccggagctg gctcttacca tcgctctcaa tcctggaggg cggcccaaag agccccattt
     3601 gcacagctac aaggaggcct tcgaggagat ggagggaacc tccccgagca gcccaccacc
     3661 cagtggggtg cggtcccccc cgggtctggc caagacaccc ctgtctgctc tgggcctgaa
     3721 acctcacaac ccagcggaca tcctgttgca ccccacagga gttaccagaa gacggattca
     3781 gccagaggaa gatgagggga aggtggtggt caggctgtca gaagagcccc ggagctatgt
     3841 ggagtctgtg gcacggacag cggtggctgg accccgagct caggactctg agcccaagag
     3901 ctttagtgct ccagccaccc aggcctatgg ccatgagata cccctgagga acgggaccct
     3961 gggtggctcc tttgtctccc ccagccccct ctccaccagc agccccatcc tcagtgctga
     4021 cagcacttca gtggggagtt tcccgtcggg agagagcagt gaccagggtc cccggacgcc
     4081 cacccagcct ctgttggagt ctggcttccg ctcaggcagc ctgggacagc ccagcccgtc
     4141 tgcccagaga aactaccaga gctcttctcc tctcccgact gtgggcagta gctacagcag
     4201 ccccgactac tcacttcagc atttcagctc ctctccagaa agccaggctc gcgctcagtt
     4261 cagtgtggct ggcgtccaca cggtgcctgg gagccctcag gcgcgccaca gaacagtggg
     4321 caccaacact ccccctagtc ctggcttcgg ctggcgggcc atcaatccca gcatggctgc
     4381 ccccagcagt cccagtttga gccatcacca gatgatgggt ccaccaggca ctggcttcca
     4441 tggtagcact gtctccagcc cccagagcag tgcagcgacc accccgggga gccccagcct
     4501 gtgtcggcac ccagcagggg tctaccaggt ttctggcctc cacaacaaag tggccaccac
     4561 cccggggagt cccagcctgg gccggcaccc tggggctcac caaggcaacc tggcctccgg
     4621 tcttcatagc aatgcaatag ccagccctgg aagccccagc ctgggccgtc acctcggagg
     4681 gtctggatct gtggttcccg gcagcccctg cttggaccgg catgtggcct atggtggcta
     4741 ttctaccccg gaggatcgga gacccacact gtcccggcag agcagtgcct ctggctacca
     4801 ggctccttcc acgccctcct tccctgtctc ccctgcctac taccctggcc tgagcagccc
     4861 tgccacctcc ccgtcaccag actccgcagc cttccggcaa gggagcccaa caccagcctt
     4921 gccagagaag cgaaggatgt cagtgggaga ccgggcaggc agcctcccca actatgccac
     4981 catcaatggg aaggtgtctt cgcctgtcgc cagcggcatg tccagtccca gtgggggcag
     5041 caccgtctcc ttctcccaca ctctgcccga cttctccaag tactccatgc cagacaacag
     5101 cccggagacg cgggctaaag tgaagtttgt ccaggacact tctaagtatt ggtacaagcc
     5161 tgagatttcc agggagcagg ccatcgcgct cctcaaggac caggagccgg gggccttcat
     5221 catccgcgac agtcactcct tccgaggcgc gtacgggctg gccatgaagg tgtcttcgcc
     5281 acctccaacc atcatgcagc agaataaaaa aggagacatg acccatgagc tggtcaggca
     5341 ttttctgata gagactggcc ccagaggagt caagctcaag ggctgcccca atgagccaaa
     5401 cttcggatcg ctgtctgccc tggtctacca gcactccatc atcccattgg ccctgccttg
     5461 caagctggtc attccaaacc gagaccccac agatgaatcg aaagatagct ccggccctgc
     5521 caactcaact gcagacctgc tgaaacaagg ggcagcctgc aatgtgctct tcgtcaactc
     5581 tgtggacatg gagtcactca ctgggccaca ggccatctct aaagccacat ctgagacgtt
     5641 ggctgcagac cccacgccag ctgccaccat cgttcacttc aaagtctctg cccagggaat
     5701 cactctgact gacaaccaga gaaagctctt tttcagacgc cactaccctc tcaacactgt
     5761 caccttctgt gacctggacc cacaggaaag aaagtggatg aaaacagagg gtggtgcccc
     5821 tgctaagctc ttcggcttcg tggcccggaa gcagggcagc accacggaca acgcctgcca
     5881 cctctttgct gagcttgacc ccaaccagcc ggcctctgcc atcgtcaact tcgtctccaa
     5941 ggtcatgctg aatgccggcc aaaagagatg aggatccacc ggatctagat aactgatcat
     6001 aatcagccat a
//
