LOCUS       sequence-85512-e        1238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 587, 
            Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1238)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      483..492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             489..1199
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(634..652)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     902..921
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggtgg gtcggaacag cgccatcgcc gccggtgtat
       61 gcggggccct tttcattggg tactgcatct acttcgaccg caaaagacga agtgacccca
      121 acttcaagaa caggcttcga gaacgaagaa agaaacagaa gcttgccaag gagagagctg
      181 ggctttccaa gttacctgac cttaaagatg ctgaagctgt tcagaagttc ttccttgaag
      241 aaatacagct tggtgaagag ttactagctc aaggtgaata tgagaagggc gtagaccatc
      301 tgacaaatgc aattgctgtg tgtggacagc cacagcagtt actgcaggtc ttacagcaaa
      361 ctcttccacc accagtgttc cagatgcttc tgactaagct cccaacaatt agtcagagaa
      421 ttgtaagtgc tcagagcttg gctgaagatg atgtggaagg cggtagcggg gatccaccgg
      481 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
      541 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      601 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      661 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      721 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      781 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      841 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      901 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      961 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     1021 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     1081 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     1141 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa
     1201 gcggccgcga ctctagatca taatcagcca taccacat
//
