LOCUS       sequence-85533-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Zyxin-C-13
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      776..785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattccatg
      781 gcggcccccc gcccgtctcc cgcgatcccc gtttcggtct cggctccggc tttttacgcc
      841 ccgcagaaga agttcggccc tgtggtggcc ccaaagccca aagtgaatcc cttccggccc
      901 agggacagcg agcctccccc ggcacccggg gcccagcgcg cacagatggg ccgggtgggc
      961 gatattcccc cgccgccccc ggaagacttt cccctgcctc cacctcccct tgctggggat
     1021 ggcgacgatg cagagggtgc tctgggaggt gccttcccgc cgccccctcc cccgatcgag
     1081 gaatcatttc cccctgcgcc tctggaggag gagatcttcc cttccccgcc gcctcctccg
     1141 gaggaggagg gagggcctga ggcccccata ccgcccccac cacagcccag ggagaaggtg
     1201 agcagtattg atttggagat cgactctctg tcctcactgc tggatgacat gaccaagaat
     1261 gatcctttca aagcccgggt gtcatctgga tatgtgcccc caccagtggc cactccattc
     1321 agttccaagt ccagtaccaa gcctgcagcc gggggcacag cacccctgcc tccttggaag
     1381 tccccttcca gctcccagcc tctgccccag gttccggctc cggctcagag ccagacacag
     1441 ttccatgttc agccccagcc ccagcccaag cctcaggtcc aactccatgt ccagtcccag
     1501 acccagcctg tgtctttggc taacacccag ccccgagggc ccccagcctc atctccggct
     1561 ccagccccta agttttctcc agtgactcct aagtttactc ctgtggcttc caagttcagt
     1621 cctggagccc caggtggatc tgggtcacaa ccaaatcaaa aattggggca ccccgaagct
     1681 ctttctgctg gcacaggctc ccctcaacct cccagcttca cctatgccca gcagagggag
     1741 aagccccgag tgcaggagaa gcagcacccc gtgcccccac cggctcagaa ccaaaaccag
     1801 gtgcgctccc ctggggcccc agggcccctg actctgaagg aggtggagga gctggagcag
     1861 ctgacccagc agctaatgca ggacatggag catcctcaga ggcagaatgt ggctgtcaac
     1921 gaactctgcg gccgatgcca tcaacccctg gcccgggcgc agccagccgt ccgcgctcta
     1981 gggcagctgt tccacatcgc ctgcttcacc tgccaccagt gtgcgcagca gctccagggc
     2041 cagcagttct acagtctgga gggggcgccg tactgcgagg gctgttacac tgacaccctg
     2101 gagaagtgta acacctgcgg ggagcccatc actgaccgca tgctgagggc cacgggcaag
     2161 gcctatcacc cgcactgctt cacctgtgtg gtctgcgccc gccccctgga gggcacctcc
     2221 ttcatcgtgg accaggccaa ccggccccac tgtgtccccg actaccacaa gcagtacgcc
     2281 ccgaggtgct ccgtctgctc tgagcccatc atgcctgagc ctggccgaga tgagactgtg
     2341 cgagtggtcg ccctggacaa gaacttccac atgaagtgtt acaagtgtga ggactgcggg
     2401 aagcccctgt cgattgaggc agatgacaat ggctgcttcc ccctggacgg tcacgtgctc
     2461 tgtcggaagt gccacactgc tagagcccag acctaagaat tctgcagtcg acggtaccgc
     2521 gggcccggga tccaccggat ctagataact gatcat
//
