LOCUS       sequence-85548-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Yamada KM
  TITLE     Yamada Lab Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FN 127
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(633..653)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(633..651)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(708..726)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      722..769
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgggtc ttgattcccc aactggcatt
       61 gacttttctg atattactgc caactctttt actgtgcact ggattgctcc tcgagccacc
      121 atcactggct acaggatccg ccatcatccc gagcacttca gtgggagacc tcgagaagat
      181 cgggtgtctc cttcagacaa ttccatcacc ctcaccaacc tcactccagg cacagagtat
      241 gtggtcagca tcgttgctct taatggcaga gaggaaagtc ccttattgat tggccaacaa
      301 tcaacagttt ctgatgttcc gagggacctg gaagttgttg ctgcgacccc caccagccta
      361 ctgatcagct gggatgctcc tgctgtcaca gtgagatatt acaggatcac ttacggagaa
      421 acaggaggaa atagccctgt ccaggagttc actgtgcctg ggagcaagtc tacagctacc
      481 atcagcggcc ttaaacctgg agttgattat accatcactg tgtatgctgt cactggccgt
      541 ggagacagcc ccgcaagcag caagccaatt tccattaatt accgaacaga aattgacaaa
      601 ccatcccaga tgtagaattc cagcttttgt tccctttagt gagggttaat tgcgcgtcaa
      661 ttcggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      721 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      781 aactatatcc ggatcgagat caattctggc gtaatagcga agaggcccgc accgatcgcc
      841 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgta
//
