LOCUS       sequence-85634-e        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 384, Emission: 450.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzurite-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(804..806,807..809,810..1523)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFRTAAGITHGMDELYK"
     primer_bind     complement(849..870)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1110..1129)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcgcgg
       61 ccgcacgctc ctcgggacac agtgccaacc atccagagga caagatggat tggggcacac
      121 tacagagcat cctcgggggt gtcaacaagc actccaccag cattgggaaa atctggctca
      181 ctgtcctctt catcttccgc atcatgatcc tcgtggtggc cgcgaaggag gtgtggggag
      241 atgagcaagc cgattttgtt tgcaacactc tccagcctgg ctgtaagaat gtgtgctacg
      301 accactactt ccccatctct cacatccggc tctgggctct gcagctgatc atggtgtcca
      361 cgccggccct cctggtagct atgcacgtgg cctaccggag acacgaaaag aaacggaagt
      421 tcatgaaggg agagataaag aacgagttta aggacatcga agagatcaaa acccagaagg
      481 tccgtatcga agggtccctg tggtggacct acaccaccag catcttcttc cgggtcatct
      541 tcgaagctgt cttcatgtat gtcttttaca tcatgtacaa tggcttcttc atgcagcgtc
      601 tggtgaagtg taacgcctgg ccttgtccca atacagtgga ctgcttcatt tccaggccca
      661 cagaaaagac tgtcttcacg gtgttcatga tctctgtgtc tggaatttgc atcctgctaa
      721 acatcacaga gctgtgctat ctgttcatta ggtattgctc agggaagtcc aaaagaccag
      781 tcgcggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
      841 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      901 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      961 agctgcccgt gccctggccc accctcgtga ccaccctgag ccacggcgtg cagtgcttca
     1021 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1081 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1141 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1201 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacatctata
     1261 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1321 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1381 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     1441 acgagaagcg cgatcacatg gtcctgctgg agttccgcac cgccgccggg atcactcacg
     1501 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     1561 tt
//
