LOCUS       sequence-85635-e        1741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 384, Emission: 450.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1741)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzurite-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(982..984,985..987,988..1701)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFRTAAGITHGMDELYK"
     primer_bind     complement(1027..1048)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1288..1307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcc ctctgggaaa
       61 gggcagcagc agccaggtgt ggcagtgaca gggaggtgtg aatgaggcag gatgaactgg
      121 acaggtttgt acaccttgct cagtggcgtg aaccggcatt ctactgccat tggccgagta
      181 tggctctcgg tcatcttcat cttcagaatc atggtgctgg tggtggctgc agagagtgtg
      241 tggggtgatg agaaatcttc cttcatctgc aacacactcc agcctggctg caacagcgtt
      301 tgctatgacc aattcttccc catctcccat gtgcggctgt ggtccctgca gctcatccta
      361 gtttccaccc cagctctcct cgtggccatg cacgtggctc accagcaaca catagagaag
      421 aaaatgctac ggcttgaggg ccatggggac cccctacacc tggaggaggt gaagaggcac
      481 aaggtccaca tctcagggac actgtggtgg acctatgtca tcagcgtggt gttccggctg
      541 ttgtttgagg ccgtcttcat gtatgtcttt tatctgctct accctggcta tgccatggtg
      601 cggctggtca agtgcgacgt ctacccctgc cccaacacag tggactgctt cgtgtcccgc
      661 cccaccgaga aaaccgtctt caccgtcttc atgctagctg cctctggcat ctgcatcatc
      721 ctcaatgtgg ccgaggtggt gtacctcatc atccgggcct gtgcccgccg agcccagcgc
      781 cgctccaatc caccttcccg caagggctcg ggcttcggcc accgcctctc acctgaatac
      841 aagcagaatg agatcaacaa gctgctgagt gagcaggatg gctccctgaa agacatactg
      901 cgccgcagcc ctggcaccgg ggctgggctg gctgaaaaga gcgaccgctg ctcggcctgc
      961 gcggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1021 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1081 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1141 ctgcccgtgc cctggcccac cctcgtgacc accctgagcc acggcgtgca gtgcttcagc
     1201 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1261 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1321 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1381 gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa catctatatc
     1441 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1501 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1561 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     1621 gagaagcgcg atcacatggt cctgctggag ttccgcaccg ccgccgggat cactcacggc
     1681 atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
     1741 g
//
