LOCUS       sequence-85639-e        4061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 384, Emission: 450.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4061)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzurite-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFRTAAGITHGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gccacggcgt
      241 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca
      481 caacatctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttccgca ccgccgccgg
      721 gatcactcac ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattcggga aactcgagaa caaagcttga aaacggccgc atgccagtgt ttcatacgcg
      841 cacgatcgag agcatcctgg agccggtggc acagcagatc tcccacctgg tgataatgca
      901 cgaggagggc gaggtggacg gcaaagccat tcctgacctc accgcgcccg tggccgccgt
      961 gcaggcggcc gtcagcaacc tcgtccgggt tggaaaagag actgttcaaa ccactgagga
     1021 tcagattttg aagagagata tgccaccagc atttattaag gttgagaatg cttgcaccaa
     1081 gcttgtccag gcagctcaga tgcttcagtc agacccttac tcagtgcctg ctcgagatta
     1141 tctaattgat gggtcaaggg gcatcctctc tggaacatca gacctgctcc ttaccttcga
     1201 tgaggctgag gtccgtaaaa ttattagagt ttgcaaagga attttggaat atcttacagt
     1261 ggcagaggtg gtggagacta tggaagattt ggtcacttac acaaagaatc ttgggccagg
     1321 aatgactaag atggccaaga tgattgacga gagacagcag gagctcactc accaggagca
     1381 ccgagtgatg ttggtgaact cgatgaacac cgtgaaagag ttgctgccag ttctcatttc
     1441 agctatgaag atttttgtaa caactaaaaa ctcaaaaaac caaggcatag aggaagcttt
     1501 aaaaaatcgc aattttactg tagaaaaaat gagtgctgaa attaatgaga taattcgtgt
     1561 gttacaactc acctcttggg atgaagatgc ctgggccagc aaggacactg aagccatgaa
     1621 gagagcattg gcctccatag actccaaact gaaccaggcc aaaggttggc tccgtgaccc
     1681 tagtgcctcc ccaggggatg ctggtgagca ggccatcaga cagatcttag atgaagctgg
     1741 aaaagttggt gaactctgtg caggcaaaga acgcagggag attctgggaa cttgcaaaat
     1801 gctagggcag atgactgatc aagtggctga cctccgtgcc agaggacaag gatcctcacc
     1861 ggtggccatg cagaaagctc agcaggtatc tcagggtctg gatgtgctca cagcaaaagt
     1921 ggaaaatgca gctcgcaagc tggaagccat gaccaactca aagcagagca ttgcaaagaa
     1981 gatcgatgct gctcagaact ggcttgcaga tccaaatggt ggaccggaag gagaagagca
     2041 gattcgaggt gctttggctg aagctcggaa aatagcagaa ttatgtgatg atcctaaaga
     2101 aagagatgac attctacgtt cccttgggga aatatctgct ctgacttcta aattagcaga
     2161 tctacgaaga caggggaaag gagattctcc agaggctcga gccttggcca aacaggtggc
     2221 cacggccctg cagaacctgc agaccaaaac caaccgggct gtggccaaca gcagaccggc
     2281 caaagcagct gtacaccttg agggcaagat tgagcaagca cagcggtgga ttgataatcc
     2341 cacagtggat gaccgtggag tcggtcaggc tgccatccgg gggcttgtgg ccgaagggca
     2401 tcgtctggct aatgttatga tggggcctta tcggcaagat cttctcgcca agtgtgaccg
     2461 agtggaccag ctgacagccc agctggctga cctggctgcc agaggggaag gggagagtcc
     2521 tcaggcacga gcacttgcat ctcagctcca agactcctta aaggatctaa aagctcggat
     2581 gcaggaggcc atgactcagg aagtgtcaga tgttttcagc gataccacaa ctcccatcaa
     2641 gctgttggca gtggcagcca cggcgcctcc tgatgcgcct aacagggaag aggtatttga
     2701 tgagagggca gctaactttg aaaaccattc aggaaagctt ggtgctacgg ccgagaaggc
     2761 ggctgcggtt ggtactgcta ataaatcaac agtggaaggc attcaggcct cagtgaagac
     2821 ggcccgagaa ctcacacccc aggtggtctc ggctgctcgt atcttactta ggaaccctgg
     2881 aaatcaagct gcttatgaac attttgagac catgaagaac cagtggatcg ataatgttga
     2941 aaaaatgaca gggctggtgg acgaagccat tgataccaaa tctctgttgg atgcttcaga
     3001 agaagcaatt aaaaaagacc tggacaagtg caaggtagct atggccaaca ttcagcctca
     3061 gatgctggtt gctggggcaa ccagtattgc tcgtcgggcc aaccggatcc tgctggtggc
     3121 taagagggag gtggagaatt ccgaggatcc caagttccgt gaggctgtga aagctgcctc
     3181 tgatgaattg agcaaaacca tctccccgat ggtgatggat gcaaaagctg tggctggaaa
     3241 catttccgac cctggactgc aaaagagctt cctggactca ggatatcgga tcctgggagc
     3301 tgtggccaag gtcagagaag ccttccaacc tcaggagcct gacttcccgc cgcctccacc
     3361 agaccttgaa caactccgac taacagatga gcttgctcct cccaaaccac ctctgcctga
     3421 aggtgaggtc cctccaccta ggcctccacc accagaggaa aaggatgaag agttccctga
     3481 gcagaaggcc ggggaggtga ttaaccagcc aatgatgatg gctgccagac agctccatga
     3541 tgaagctcgc aaatggtcca gcaagggcaa tgacatcatt gcagcagcca agcgcatggc
     3601 tctgctgatg gctgagatgt ctcggctggt aagagggggc agtggtacca agcgggcact
     3661 cattcagtgt gccaaggaca tcgccaaggc ctcagatgag gtgactcggt tggccaagga
     3721 ggttgccaag cagtgcacag ataaacggat tagaaccaac ctcttacagg tatgtgagcg
     3781 aatcccaacc ataagcaccc agctcaaaat cctgtccaca gtgaaggcca ccatgctggg
     3841 ccggaccaac atcagtgatg aggagtctga gcaggccaca gagatgctgg ttcacaatgc
     3901 ccagaacctc atgcagtctg tgaaggagac tgtgcgggaa gctgaagctg cttcaatcaa
     3961 aattcgaaca gatgctggat ttacactgcg ctgggttaga aagactccct ggtaccagta
     4021 ggcggccgcg actctagatc ataatcagcc ataccacatt g
//
