LOCUS       sequence-85682-e        3549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 399, 
            Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..739
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    2558..2569
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 accgtcagat ccgctagcgg tggccaccat ggtgtctaag ggcgaagagc tgattaagga
       61 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
      121 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
      181 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
      241 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
      301 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
      361 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
      421 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
      481 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
      541 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
      601 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
      661 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
      721 actggggcac aagcttaatt ccggactcag atctggcagc ggtggaggct tggaggagcc
      781 ggaggccgcg acgcggacag ccgcggccgt ggattgcaaa gacagacccg ggttcccagt
      841 gaagaggtta atacaagccc gcttgccctt caaacgcctg aaccttgttc ccaaggagaa
      901 agttgaggaa gacacatcac caaaagcagc tgtggaaagc aaagtcccgg atctacaatt
      961 gtctttgggc acttttgaaa gccagtgtca cacaggctct cacgtaggct tgagtacaaa
     1021 gttggtcggt ggccagggcc ccattgatag cttcttgagg gcaacaatca agccagtacc
     1081 gagtgtggtc attatcgacc tgacggagaa ctgcagtgac attccagaca gccctgaggg
     1141 ccacagtgaa ctaagtcctg acactgctgg tgtggtcacc acagtggaag gagctgccaa
     1201 gcagcaggag cactctgcag cagagctgtg tctcctagag accccttcag acatcacttg
     1261 tcacatggaa gaggagcctg ggagtccagg agacccaaag aggacgggtg actgtcaggc
     1321 tggttcacta cagagctgtc cggagctgac cccaggttct aggacatgcc ccaccaagga
     1381 gttatcaagc tggagcaagg caggggatct cctatttata gagaaagtgc ctgtggtggt
     1441 gctagaggac atcctggcca ctaagccctc cattgcctct cttcccatga tgtccctgga
     1501 taggagtgtc acttcagaga gtgaaatact ggagtcctgc cccgaggatg actccatact
     1561 gagccactcc tccaccaact cctcttctcc aaccagctct cctgagggac catccacacc
     1621 cccagaacat cggggtggga gaagctcccc ctccacccca gcctgcagag ttgccaagaa
     1681 ctttgtcaaa ggctccacag agaagggcag gagcaaactg cacagagata gagaacagca
     1741 gagggaagag aaggaaaagc tgagggaaga gatacgacga gccaaggagg aggcacggaa
     1801 gaagaaggag gaggagaagg agctgaagga aaaggagcgg agggaaaagc gggagaaaga
     1861 tgagaaggag aaggcggaga agcagcggct aaaggaggaa aaacgcaagg agcggcagga
     1921 agctctggag gctaaactgg aggagaagag gaagaaggag gaagagaagc ggttacggga
     1981 ggaagagaag cggttacggg aagaagagaa gcgcattaag gcagaaaagg cagagatcac
     2041 tcgattcttc cagaaaccga agactccgca ggctcccaag accctggccg gttcctgtgg
     2101 taagtttgcc ccctttgaaa tcaaagaaca catggtgctg gctccacggt gccgagctgc
     2161 cttggatcaa gacctgtgcg atcagctgga ccagctcctg cagcagcaga gtgtggcaag
     2221 caccttcctc agtgacctaa aaagccggct gcccttgaga tcggggccca cccgggtctg
     2281 tggccatgac acagacatca tgaacaggga tgtggtcatt gtggagagca gcaaggtgga
     2341 cggtgtgtct gaaaggaaga agtttggccg catgaagctt ctgcagttct cagagaacca
     2401 ccggccagca tactggggga cgtggaacaa gaagacagcc atcatccgcc cccggaaccc
     2461 ctgggcccag gacaaagacc ttcttgacta tgaggtagac agtgacgatg agtgggaaga
     2521 ggaggaacct ggggagtctt tgtcccacag tgagggggat gaggatgacg atgtggggga
     2581 agatgaggat gaggacgatg gcttctttgt gccccatggg tacttatctg aagatgaagg
     2641 cgtgactgag gagtgtgcag accctgagaa ccacaaagta caccagaagc tgaaggccaa
     2701 ggagtgggat gagctcctag ccaagggcaa gcgctttcgt gtgctgcagc ctgtgcacgt
     2761 gggatgtgta tgggcatcag aggcagccaa ctgcacgagt tccgacttga agctgctgca
     2821 gcagttcact gcatgcctgc tggatgtggc atcccctgat gagccagaac ccggggcctc
     2881 caggagggaa aagagggacc agcatatcct ggcccagttg cttccgctgc tacatggcaa
     2941 tgtgaatggg agcaaggtga tcatccatga attccaggag caatgccgca ggggcctgct
     3001 gacccttcca agtcccaccc cacacctgca gatgcccaac ttggaggatg ctgttgctgt
     3061 gccatccaag gccaggctga agcgccttat ctctgagaac tctgcctatg agaagcggcc
     3121 caacttccgt atgtgctggt atgtgcaccc agaggtgctc aagagctttg gccaggagtg
     3181 cctgccagtg ccctgccagt ggacctacat caccaccatg ccctcagcac ccagggaaga
     3241 cagtggcagt gcttctactg aggggcctgg ccagagcaca cctatgctac tcaagcggaa
     3301 gccagcggcc accatgtgca tcacgcagtt tatgaagaag cggcgctatg atggacaggt
     3361 tgggtctggg gacatggatg gcttccaggc agacacagaa gaggatgaag aggatgacac
     3421 agactgtatg atcatagatg tgccagatgt tgggagtgac gtgtcagaag ctcccatacc
     3481 tgctcccaca ctttgcaagt gaggatccac cggatctaga taactgatca taatcagcca
     3541 taccacatt
//
