LOCUS       sequence-85683-e        2576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1845..1854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1851..2561
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 tagcgctacc ggactcagat ctcgagaccg ccatggaagg gaagtggttg ctgtgtatgt
       61 tactggtgct tggaactgct attgttgagg ctcatgatgg acatgatgat gatgtgattg
      121 atattgagga tgaccttgac gatgtcattg aagaggtaga agactcaaaa ccagatacca
      181 ctgctcctcc ttcatctccc aaggttactt acaaagctcc agttccaaca ggggaagtat
      241 attttgctga ttcttttgac agaggaactc tgtcagggtg gattttatcc aaagccaaga
      301 aagacgatac cgatgatgaa attgccaaat atgatggaaa gtgggaggta gaggaaatga
      361 aggagtcaaa gcttccaggt gataaaggac ttgtgttgat gtctcgggcc aagcatcatg
      421 ccatctctgc taaactgaac aagcccttcc tgtttgacac caagcctctc attgttcagt
      481 atgaggttaa tttccaaaat ggaatagaat gtggtggtgc ctatgtgaaa ctgctttcta
      541 aaacaccaga actcaacctg gatcagttcc atgacaagac cccttatacg attatgtttg
      601 gtccagataa atgtggagag gactataaac tgcacttcat cttccgacac aaaaacccca
      661 aaacgggtat ctatgaagaa aaacatgcta agaggccaga tgcagatctg aagacctatt
      721 ttactgataa gaaaacacat ctttacacac taatcttgaa tccagataat agttttgaaa
      781 tactggttga ccaatctgtg gtgaatagtg gaaatctgct caatgacatg actcctcctg
      841 taaatccttc acgtgaaatt gaggacccag aagaccggaa gcccgaggat tgggatgaaa
      901 gaccaaaaat cccagatcca gaagctgtca agccagatga ctgggatgaa gatgcccctg
      961 ctaagattcc agatgaagag gccacaaaac ccgaaggctg gttagatgat gagcctgagt
     1021 acgtacctga tccagacgca gagaaacctg aggattggga tgaagacatg gatggagaat
     1081 gggaggctcc tcagattgcc aaccctagat gtgagtcagc tcctggatgt ggtgtctggc
     1141 agcgacctgt gattgacaac cccaattata aaggcaaatg gaagcctcct atgattgaca
     1201 atcccagtta ccagggaatc tggaaaccca ggaaaatacc aaatccagat ttctttgaag
     1261 atctggaacc tttcagaatg actcctttta gtgctattgg tttggagctg tggtccatga
     1321 cctctgacat tttttttgac aactttatca tttgtgctga tcgaagaata gttgatgatt
     1381 gggccaatga tggatggggc ctgaagaaag ctgctgatgg ggctgctgag ccaggcgttg
     1441 tggggcagat gaacgaggca gctgaagagc gcccgtggct gtgggtagtc tatattctaa
     1501 ctgtagccct tcctgtgttc ctggttatcc tcttctgctg ttctggaaag aaacagacca
     1561 gtggtatgga gtataagaaa actgatgcac ctcaaccgga tgtgaaggaa gaggaagaag
     1621 agaaggaaga ggaaaaggac aagggagatg aggaggagga aggagaagag aaacttgaag
     1681 agaaacagaa aagtgatgct gaagaagatg gtggcactgt cagtcaagag gaggaagaca
     1741 gaaaacctaa agcagaggag gatgaaattt tgaacagatc accaagaaac agaaagccac
     1801 gaagagaggg gggtgcgtca ggcggcagcg gatctggacc ggtcgccacc atggtgtcta
     1861 agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     1921 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     1981 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     2041 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     2101 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     2161 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     2221 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     2281 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     2341 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc acatatagat
     2401 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     2461 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     2521 gatactgcga cctccctagc aaactggggc acaagcttaa ttaagcggcc gcgact
//
