LOCUS       sequence-85686-e        1566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-CD81-10
FEATURES             Location/Qualifiers
     source          1..1566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..735
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 tcagatccgc tagcggtggc caccatggtg tctaagggcg aagagctgat taaggagaac
       61 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
      121 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
      181 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
      241 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
      301 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
      361 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
      421 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
      481 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
      541 catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa gaacctcaag
      601 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
      661 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
      721 gggcacaagc ttaattccgg actcagatct cgagctcaag cttccggagt ggagggctgc
      781 accaagtgca tcaagtacct gctcttcgtc ttcaatttcg tcttctggct ggctggaggc
      841 gtgatcctgg gtgtggccct gtggctccgc catgacccgc agaccaccaa cctcctgtat
      901 ctggagctgg gagacaagcc cgcgcccaac accttctatg taggcatcta catcctcatc
      961 gctgtgggcg ctgtcatgat gttcgttggc ttcctgggct gctacggggc catccaggaa
     1021 tcccagtgcc tgctggggac gttcttcacc tgcctggtca tcctgtttgc ctgtgaggtg
     1081 gccgccggca tctggggctt tgtcaacaag gaccagatcg ccaaggatgt gaagcagttc
     1141 tatgaccagg ccctacagca ggccgtggtg gatgatgacg ccaacaacgc caaggctgtg
     1201 gtgaagacct tccacgagac gcttgactgc tgtggctcca gcacactgac tgctttgacc
     1261 acctcagtgc tcaagaacaa tttgtgtccc tcgggcagca acatcatcag caacctcttc
     1321 aaggaggact gccaccagaa gatcgatgac ctcttctccg ggaagctgta cctcatcggc
     1381 attgctgcca tcgtggtcgc tgtgatcatg atcttcgaga tgatcctgag catggtgctg
     1441 tgctgtggca tccggaacag ctccgtgtac tgaggcggat ccaagggcga attctgcagt
     1501 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac
     1561 cacatt
//
