LOCUS       sequence-85690-e        2414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..98
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1671..1680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1677..2387
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcatgg
       61 ggagcagcaa gagcaagccc aaggacccca gccagcgccg gcgcagcctg gagccacccg
      121 acagcaccca ccacggggga ttcccagcct cgcagacccc caacaagaca gcagcccccg
      181 acacgcaccg cacccccagc cgctcctttg ggaccgtggc caccgagccc aagctcttcg
      241 ggggcttcaa cacttctgac accgtcacgt cgccgcagcg tgccggggca ctggctggcg
      301 gcgtcaccac tttcgtggct ctctatgact acgagtcccg gactgaaacg gacttgtcct
      361 tcaagaaagg agaacgcctg cagattgtca acaacacgga aggtgactgg tggctggctc
      421 attccctcac tacaggacag acgggctaca tccccagtaa ctatgtcgcg ccctcagact
      481 ccatccaggc tgaagagtgg tactttggga agatcactcg tcgggagtcc gagcggctgc
      541 tgctcaaccc cgaaaacccc cggggaacct tcttggtccg ggagagcgag acgacaaaag
      601 gtgcctattg cctctccgtt tctgactttg acaacgccaa ggggctcaat gtgaagcact
      661 acaagatccg caagctggac agcggcggct tctacatcac ctcacgcaca cagttcagca
      721 gcctgcagca gctggtggcc tactactcca aacatgctga tggcttgtgc caccgcctga
      781 ccaacgtctg ccccacgtcc aagccccaga cccagggact cgccaaggac gcgtgggaaa
      841 tcccccggga gtcgctgcgg ctggaggtga agctggggca gggctgcttt ggagaggtct
      901 ggatggggac ctggaacggc accaccagag tggccataaa gactctgaag cccggcacca
      961 tgtccccgga ggccttcctg caggaagccc aagtgatgaa gaagctccgg catgagaagc
     1021 tggttcagct gtacgcagtg gtgtcggaag agcccatcta catcgtcact gagtacatga
     1081 gcaaggggag cctcctggat ttcctgaagg gagagatggg caagtacctg cggctgccac
     1141 agctcgtcga tatggctgct cagattgcat ccggcatggc ctatgtggag aggatgaact
     1201 acgtgcaccg agacctgcgg gcggccaaca tcctggtggg ggagaacctg gtgtgcaagg
     1261 tggctgactt tgggctggca cgcctcatcg aggacaacga gtacacagca cggcaaggtg
     1321 ccaagttccc catcaagtgg acagcccccg aggcagccct ctatggccgg ttcaccatca
     1381 agtcggatgt ctggtccttc ggcatcctgc tgactgagct gaccaccaag ggccgggtgc
     1441 catacccagg gatggtcaac agggaggtgc tggaccaggt ggagaggggc taccgcatgc
     1501 cctgcccgcc cgagtgcccc gagtcgctgc atgacctcat gtgccagtgc tggcggaagg
     1561 accctgagga gcggcccact tttgagtacc tgcaggcctt cctggaggac tacttcacct
     1621 cgacagagcc ccagtaccag cctggagaga acctacggga tccaccggtc gccaccatgg
     1681 tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca
     1741 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     1801 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     1861 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
     1921 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     1981 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     2041 acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac
     2101 tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa
     2161 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc aagaccacat
     2221 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
     2281 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
     2341 tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattaa gcggccgcga
     2401 ctctagatca taat
//
