LOCUS       sequence-85730-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             229..939
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcgccaccat gattcacacc
       61 aacctgaaga aaaagttcag ctgctgcgtc ctggtctttc ttctgtttgc agtcatctgt
      121 gtgtggaagg aaaagaagaa agggagttac tatgattcct ttaaattgca aaccaaggaa
      181 ttcctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgtctaag
      241 ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
      301 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      361 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
      421 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
      481 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      541 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      601 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
      661 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
      721 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
      781 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
      841 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
      901 tactgcgacc tccctagcaa actggggcac aagcttaatt aagcggccgc gactctagat
      961 cataatcagc cataccacat t
//
